%0 Journal Article
%A Li, Kaizhen
%A Figarella, Katherine
%A Su, Xin
%A Kovalchuk, Yury
%A Gorzolka, Jessika
%A Neher, Jonas J
%A Mojtahedi, Nima
%A Casadei, Nicolas
%A Hedrich, Ulrike B S
%A Garaschuk, Olga
%T Endogenous but not sensory-driven activity controls migration, morphogenesis and survival of adult-born juxtaglomerular neurons in the mouse olfactory bulb.
%J Cellular and molecular life sciences
%V 80
%N 4
%@ 1420-682X
%C Cham (ZG)
%I Springer International Publishing AG
%M DZNE-2023-00363
%P 98
%D 2023
%Z CC BY
%X The development and survival of adult-born neurons are believed to be driven by sensory signaling. Here, in vivo analyses of motility, morphology and Ca2+ signaling, as well as transcriptome analyses of adult-born juxtaglomerular cells with reduced endogenous excitability (via cell-specific overexpression of either Kv1.2 or Kir2.1 K+ channels), revealed a pronounced impairment of migration, morphogenesis, survival, and functional integration of these cells into the mouse olfactory bulb, accompanied by a reduction in cytosolic Ca2+ fluctuations, phosphorylation of CREB and pCREB-mediated gene expression. Moreover, K+ channel overexpression strongly downregulated genes involved in neuronal migration, differentiation, and morphogenesis and upregulated apoptosis-related genes, thus locking adult-born cells in an immature and vulnerable state. Surprisingly, cells deprived of sensory-driven activity developed normally. Together, the data reveal signaling pathways connecting the endogenous intermittent neuronal activity/Ca2+ fluctuations as well as enhanced Kv1.2/Kir2.1 K+ channel function to migration, maturation, and survival of adult-born neurons.
%K Mice
%K Animals
%K Olfactory Bulb: metabolism
%K Neurons: metabolism
%K Neurogenesis: genetics
%K Cell Differentiation
%K Cell Movement
%K Adult neurogenesis (Other)
%K Differentiation (Other)
%K Endogenous activity (Other)
%K Migration (Other)
%K Neuronal development (Other)
%K Olfactory bulb (Other)
%K Potassium channels (Other)
%K Spontaneous calcium transients (Other)
%K Survival (Other)
%K pCREB (Other)
%F PUB:(DE-HGF)16
%9 Journal Article
%$ pmid:36932186
%2 pmc:PMC10023654
%R 10.1007/s00018-023-04753-4
%U https://pub.dzne.de/record/256611