TY  - JOUR
AU  - Li, Kaizhen
AU  - Figarella, Katherine
AU  - Su, Xin
AU  - Kovalchuk, Yury
AU  - Gorzolka, Jessika
AU  - Neher, Jonas J
AU  - Mojtahedi, Nima
AU  - Casadei, Nicolas
AU  - Hedrich, Ulrike B S
AU  - Garaschuk, Olga
TI  - Endogenous but not sensory-driven activity controls migration, morphogenesis and survival of adult-born juxtaglomerular neurons in the mouse olfactory bulb.
JO  - Cellular and molecular life sciences
VL  - 80
IS  - 4
SN  - 1420-682X
CY  - Cham (ZG)
PB  - Springer International Publishing AG
M1  - DZNE-2023-00363
SP  - 98
PY  - 2023
N1  - CC BY
AB  - The development and survival of adult-born neurons are believed to be driven by sensory signaling. Here, in vivo analyses of motility, morphology and Ca2+ signaling, as well as transcriptome analyses of adult-born juxtaglomerular cells with reduced endogenous excitability (via cell-specific overexpression of either Kv1.2 or Kir2.1 K+ channels), revealed a pronounced impairment of migration, morphogenesis, survival, and functional integration of these cells into the mouse olfactory bulb, accompanied by a reduction in cytosolic Ca2+ fluctuations, phosphorylation of CREB and pCREB-mediated gene expression. Moreover, K+ channel overexpression strongly downregulated genes involved in neuronal migration, differentiation, and morphogenesis and upregulated apoptosis-related genes, thus locking adult-born cells in an immature and vulnerable state. Surprisingly, cells deprived of sensory-driven activity developed normally. Together, the data reveal signaling pathways connecting the endogenous intermittent neuronal activity/Ca2+ fluctuations as well as enhanced Kv1.2/Kir2.1 K+ channel function to migration, maturation, and survival of adult-born neurons.
KW  - Mice
KW  - Animals
KW  - Olfactory Bulb: metabolism
KW  - Neurons: metabolism
KW  - Neurogenesis: genetics
KW  - Cell Differentiation
KW  - Cell Movement
KW  - Adult neurogenesis (Other)
KW  - Differentiation (Other)
KW  - Endogenous activity (Other)
KW  - Migration (Other)
KW  - Neuronal development (Other)
KW  - Olfactory bulb (Other)
KW  - Potassium channels (Other)
KW  - Spontaneous calcium transients (Other)
KW  - Survival (Other)
KW  - pCREB (Other)
LB  - PUB:(DE-HGF)16
C6  - pmid:36932186
C2  - pmc:PMC10023654
DO  - DOI:10.1007/s00018-023-04753-4
UR  - https://pub.dzne.de/record/256611
ER  -