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000257328 0247_ $$2doi$$a10.1002/anie.202218078
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000257328 037__ $$aDZNE-2023-00397
000257328 041__ $$aEnglish
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000257328 1001_ $$0P:(DE-2719)2812808$$aPantoja Rivillas, Christian$$b0$$eFirst author$$udzne
000257328 245__ $$aDetermining the Physico-Chemical Composition of Biomolecular Condensates from Spatially-Resolved NMR.
000257328 260__ $$aWeinheim$$bWiley-VCH$$c2023
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000257328 520__ $$aLiquid-Liquid phase separation has emerged as fundamental process underlying the formation of biomolecular condensates. Insights into the composition and structure of biomolecular condensates is, however, complicated by their molecular complexity and dynamics. Here, we introduce an improved spatially-resolved NMR experiment that enables quantitative analysis of the physico-chemical composition of multi-component biomolecular condensates in equilibrium and label-free. Application of spatially-resolved NMR to condensates formed by the Alzheimer's disease-associated protein Tau demonstrates decreased water content, exclusion of the molecular crowding agent dextran, presence of a specific chemical environment of the small molecule DSS, and ≈150-fold increased concentration of Tau inside the condensate. The results suggest that spatially-resolved NMR can have a major impact in understanding the composition and physical chemistry of biomolecular condensates.
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000257328 650_2 $$2MeSH$$aHumans
000257328 650_2 $$2MeSH$$a14-3-3 Proteins
000257328 650_2 $$2MeSH$$aAlzheimer Disease
000257328 650_2 $$2MeSH$$aBiomolecular Condensates
000257328 650_2 $$2MeSH$$aChemistry, Physical
000257328 650_2 $$2MeSH$$aMagnetic Resonance Imaging
000257328 650_2 $$2MeSH$$aChemical Phenomena
000257328 650_7 $$2NLM Chemicals$$a14-3-3 Proteins
000257328 650_7 $$2Other$$aBiomolecular condensates
000257328 650_7 $$2Other$$aMulticomponent quantification
000257328 650_7 $$2Other$$aPhase diagram
000257328 650_7 $$2Other$$aSpatially-resolved NMR
000257328 650_7 $$2Other$$aTau protein
000257328 7001_ $$0P:(DE-2719)2812657$$aIbáñez de Opakua, Alain$$b1$$udzne
000257328 7001_ $$0P:(DE-2719)2812201$$aCima Omori, Maria Sol$$b2$$udzne
000257328 7001_ $$0P:(DE-2719)2810591$$aZweckstetter, Markus$$b3$$eLast author
000257328 773__ $$0PERI:(DE-600)2011836-3$$a10.1002/anie.202218078$$gVol. 62, no. 17, p. e202218078$$n17$$pe202218078$$tAngewandte Chemie / International edition$$v62$$x1433-7851$$y2023
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