TY  - JOUR
AU  - Pantoja Rivillas, Christian
AU  - Ibáñez de Opakua, Alain
AU  - Cima Omori, Maria Sol
AU  - Zweckstetter, Markus
TI  - Determining the Physico-Chemical Composition of Biomolecular Condensates from Spatially-Resolved NMR.
JO  - Angewandte Chemie / International edition
VL  - 62
IS  - 17
SN  - 1433-7851
CY  - Weinheim
PB  - Wiley-VCH
M1  - DZNE-2023-00397
SP  - e202218078
PY  - 2023
N1  - CC BY-NC-ND
AB  - Liquid-Liquid phase separation has emerged as fundamental process underlying the formation of biomolecular condensates. Insights into the composition and structure of biomolecular condensates is, however, complicated by their molecular complexity and dynamics. Here, we introduce an improved spatially-resolved NMR experiment that enables quantitative analysis of the physico-chemical composition of multi-component biomolecular condensates in equilibrium and label-free. Application of spatially-resolved NMR to condensates formed by the Alzheimer's disease-associated protein Tau demonstrates decreased water content, exclusion of the molecular crowding agent dextran, presence of a specific chemical environment of the small molecule DSS, and ≈150-fold increased concentration of Tau inside the condensate. The results suggest that spatially-resolved NMR can have a major impact in understanding the composition and physical chemistry of biomolecular condensates.
KW  - Humans
KW  - 14-3-3 Proteins
KW  - Alzheimer Disease
KW  - Biomolecular Condensates
KW  - Chemistry, Physical
KW  - Magnetic Resonance Imaging
KW  - Chemical Phenomena
KW  - 14-3-3 Proteins (NLM Chemicals)
KW  - Biomolecular condensates (Other)
KW  - Multicomponent quantification (Other)
KW  - Phase diagram (Other)
KW  - Spatially-resolved NMR (Other)
KW  - Tau protein (Other)
LB  - PUB:(DE-HGF)16
C6  - pmid:36847235
DO  - DOI:10.1002/anie.202218078
UR  - https://pub.dzne.de/record/257328
ER  -