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@ARTICLE{PantojaRivillas:257328,
      author       = {Pantoja Rivillas, Christian and Ibáñez de Opakua, Alain
                      and Cima Omori, Maria Sol and Zweckstetter, Markus},
      title        = {{D}etermining the {P}hysico-{C}hemical {C}omposition of
                      {B}iomolecular {C}ondensates from {S}patially-{R}esolved
                      {NMR}.},
      journal      = {Angewandte Chemie / International edition},
      volume       = {62},
      number       = {17},
      issn         = {1433-7851},
      address      = {Weinheim},
      publisher    = {Wiley-VCH},
      reportid     = {DZNE-2023-00397},
      pages        = {e202218078},
      year         = {2023},
      note         = {CC BY-NC-ND},
      abstract     = {Liquid-Liquid phase separation has emerged as fundamental
                      process underlying the formation of biomolecular
                      condensates. Insights into the composition and structure of
                      biomolecular condensates is, however, complicated by their
                      molecular complexity and dynamics. Here, we introduce an
                      improved spatially-resolved NMR experiment that enables
                      quantitative analysis of the physico-chemical composition of
                      multi-component biomolecular condensates in equilibrium and
                      label-free. Application of spatially-resolved NMR to
                      condensates formed by the Alzheimer's disease-associated
                      protein Tau demonstrates decreased water content, exclusion
                      of the molecular crowding agent dextran, presence of a
                      specific chemical environment of the small molecule DSS, and
                      ≈150-fold increased concentration of Tau inside the
                      condensate. The results suggest that spatially-resolved NMR
                      can have a major impact in understanding the composition and
                      physical chemistry of biomolecular condensates.},
      keywords     = {Humans / 14-3-3 Proteins / Alzheimer Disease / Biomolecular
                      Condensates / Chemistry, Physical / Magnetic Resonance
                      Imaging / Chemical Phenomena / 14-3-3 Proteins (NLM
                      Chemicals) / Biomolecular condensates (Other) /
                      Multicomponent quantification (Other) / Phase diagram
                      (Other) / Spatially-resolved NMR (Other) / Tau protein
                      (Other)},
      cin          = {AG Zweckstetter},
      ddc          = {540},
      cid          = {I:(DE-2719)1410001},
      pnm          = {352 - Disease Mechanisms (POF4-352)},
      pid          = {G:(DE-HGF)POF4-352},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:36847235},
      doi          = {10.1002/anie.202218078},
      url          = {https://pub.dzne.de/record/257328},
}