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001     257328
005     20240112171718.0
024 7 _ |a 10.1002/anie.202218078
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024 7 _ |a pmid:36847235
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024 7 _ |a 1433-7851
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024 7 _ |a 0570-0833
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024 7 _ |a 1521-3773
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037 _ _ |a DZNE-2023-00397
041 _ _ |a English
082 _ _ |a 540
100 1 _ |a Pantoja Rivillas, Christian
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245 _ _ |a Determining the Physico-Chemical Composition of Biomolecular Condensates from Spatially-Resolved NMR.
260 _ _ |a Weinheim
|c 2023
|b Wiley-VCH
336 7 _ |a article
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500 _ _ |a CC BY-NC-ND
520 _ _ |a Liquid-Liquid phase separation has emerged as fundamental process underlying the formation of biomolecular condensates. Insights into the composition and structure of biomolecular condensates is, however, complicated by their molecular complexity and dynamics. Here, we introduce an improved spatially-resolved NMR experiment that enables quantitative analysis of the physico-chemical composition of multi-component biomolecular condensates in equilibrium and label-free. Application of spatially-resolved NMR to condensates formed by the Alzheimer's disease-associated protein Tau demonstrates decreased water content, exclusion of the molecular crowding agent dextran, presence of a specific chemical environment of the small molecule DSS, and ≈150-fold increased concentration of Tau inside the condensate. The results suggest that spatially-resolved NMR can have a major impact in understanding the composition and physical chemistry of biomolecular condensates.
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650 _ 2 |a Humans
|2 MeSH
650 _ 2 |a 14-3-3 Proteins
|2 MeSH
650 _ 2 |a Alzheimer Disease
|2 MeSH
650 _ 2 |a Biomolecular Condensates
|2 MeSH
650 _ 2 |a Chemistry, Physical
|2 MeSH
650 _ 2 |a Magnetic Resonance Imaging
|2 MeSH
650 _ 2 |a Chemical Phenomena
|2 MeSH
650 _ 7 |a 14-3-3 Proteins
|2 NLM Chemicals
650 _ 7 |a Biomolecular condensates
|2 Other
650 _ 7 |a Multicomponent quantification
|2 Other
650 _ 7 |a Phase diagram
|2 Other
650 _ 7 |a Spatially-resolved NMR
|2 Other
650 _ 7 |a Tau protein
|2 Other
700 1 _ |a Ibáñez de Opakua, Alain
|0 P:(DE-2719)2812657
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700 1 _ |a Cima Omori, Maria Sol
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700 1 _ |a Zweckstetter, Markus
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773 _ _ |a 10.1002/anie.202218078
|g Vol. 62, no. 17, p. e202218078
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|p e202218078
|t Angewandte Chemie / International edition
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|y 2023
|x 1433-7851
856 4 _ |u https://pub.dzne.de/record/257328/files/DZNE-2023-00397%20SUP.pdf
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910 1 _ |a Deutsches Zentrum für Neurodegenerative Erkrankungen
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