TY  - JOUR
AU  - Brahimi, Yacine
AU  - Knauer, Beate
AU  - Price, Alan Tobias
AU  - Valero-Aracama, Maria Jesus
AU  - Reboreda, Antonio
AU  - Sauvage, Magdalena
AU  - Yoshida, Motoharu
TI  - Persistent Firing in Hippocampal CA1 Pyramidal Cells in Young and Aged Rats.
JO  - eNeuro
VL  - 10
IS  - 3
SN  - 2373-2822
CY  - Washington, DC
PB  - Soc.
M1  - DZNE-2023-00408
SP  - ENEURO.0479-22.2023
PY  - 2023
N1  - CC BY
AB  - Persistent neuronal firing is often observed in working memory and temporal association tasks both in humans and animals, and is believed to retain necessary information in these tasks. We have reported that hippocampal CA1 pyramidal cells are able to support persistent firing through intrinsic mechanisms in the presence of cholinergic agonists. However, it still remains largely unknown how persistent firing is affected by the development of animals and aging. Using in vitro patch-clamp recordings from CA1 pyramidal cells in rat brain slices, we first show that the cellular excitability of these aged rats was significantly lower than that of the young rats, responding with fewer spikes to current injection. In addition, we found age-dependent modulations of input resistance, membrane capacitance, and spike width. However, persistent firing in aged (approximately two-year-old) rats was as strong as that in young animals, and the properties of persistent firing were very similar among different age groups. In addition, medium spike afterhyperpolarization potential (mAHP), was not increased by aging and did not correlate with the strength of persistent firing. Lastly, we estimated the depolarization current induced by the cholinergic activation. This current was proportional to the increased membrane capacitance of the aged group and was inversely correlated with their intrinsic excitability. These observations indicate that robust persistent firing can be maintained in aged rats despite reduced excitability, because of the increased amount of cholinergically induced positive current.
KW  - Humans
KW  - Rats
KW  - Animals
KW  - Child, Preschool
KW  - Pyramidal Cells: physiology
KW  - Hippocampus: physiology
KW  - Action Potentials: physiology
KW  - Neurons
KW  - Cholinergic Agents
KW  - afterhyperpolarization (Other)
KW  - aged rats (Other)
KW  - cholinergic agonist (Other)
KW  - depolarization current (Other)
KW  - hippocampus (Other)
KW  - persistent firing (Other)
KW  - Cholinergic Agents (NLM Chemicals)
LB  - PUB:(DE-HGF)16
C6  - pmid:36894320
C2  - pmc:PMC10062487
DO  - DOI:10.1523/ENEURO.0479-22.2023
UR  - https://pub.dzne.de/record/257339
ER  -