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@ARTICLE{Timmermann:257561,
author = {Timmermann, Aline and Tascio, Dario and Jabs, Ronald and
Boehlen, Anne and Domingos, Catia and Skubal, Magdalena and
Huang, Wenhui and Kirchhoff, Frank and Henneberger,
Christian and Bilkei-Gorzo, Andras and Seifert, Gerald and
Steinhäuser, Christian},
title = {{D}ysfunction of {NG}2 glial cells affects neuronal
plasticity and behavior.},
journal = {Glia},
volume = {71},
number = {6},
issn = {0894-1491},
address = {Bognor Regis [u.a.]},
publisher = {Wiley-Liss},
reportid = {DZNE-2023-00441},
pages = {1481 - 1501},
year = {2023},
abstract = {NG2 glia represents a distinct type of macroglial cells in
the CNS and is unique among glia because they receive
synaptic input from neurons. They are abundantly present in
white and gray matter. While the majority of white matter
NG2 glia differentiates into oligodendrocytes, the
physiological impact of gray matter NG2 glia and their
synaptic input are still ill defined. Here, we asked whether
dysfunctional NG2 glia affect neuronal signaling and
behavior. We generated mice with inducible deletion of the
K+ channel Kir4.1 in NG2 glia and performed comparative
electrophysiological, immunohistochemical, molecular and
behavioral analyses. Kir4.1 was deleted at postnatal day
23-26 (recombination efficiency about $75\%)$ and mice were
investigated 3-8 weeks later. Notably, these mice with
dysfunctional NG2 glia demonstrated improved spatial memory
as revealed by testing new object location recognition while
working and social memory remained unaffected. Focussing on
the hippocampus, we found that loss of Kir4.1 potentiated
synaptic depolarizations of NG2 glia and stimulated the
expression of myelin basic protein while proliferation and
differentiation of hippocampal NG2 glia remained largely
unaffected. Mice with targeted deletion of the K+ channel in
NG2 glia showed impaired long-term potentiation at CA3-CA1
synapses, which could be fully rescued by extracellular
application of a TrkB receptor agonist. Our data demonstrate
that proper NG2 glia function is important for normal brain
function and behavior.},
keywords = {Mice / Animals / Proteoglycans: metabolism / Neuroglia:
metabolism / Neurons: metabolism / Oligodendroglia:
metabolism / Neuronal Plasticity / Antigens: metabolism /
Kir4.1 (Other) / NG2 glia (Other) / myelination (Other) /
neuron-glia signaling (Other) / neuronal plasticity (Other)
/ Proteoglycans (NLM Chemicals) / Antigens (NLM Chemicals)},
cin = {AG Henneberger},
ddc = {610},
cid = {I:(DE-2719)1013029},
pnm = {351 - Brain Function (POF4-351)},
pid = {G:(DE-HGF)POF4-351},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:36802096},
doi = {10.1002/glia.24352},
url = {https://pub.dzne.de/record/257561},
}