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@ARTICLE{Toda:257694,
author = {Toda, Tomohisa and Hsu, Jonathan Y and Linker, Sara B and
Hu, Lauren and Schafer, Simon T and Mertens, Jerome and
Jacinto, Filipe V and Hetzer, Martin W and Gage, Fred H},
title = {{N}up153 {I}nteracts with {S}ox2 to {E}nable {B}imodal
{G}ene {R}egulation and {M}aintenance of {N}eural
{P}rogenitor {C}ells.},
journal = {Cell stem cell},
volume = {21},
number = {5},
issn = {1934-5909},
address = {Amsterdam [u.a.]},
publisher = {Elsevier},
reportid = {DZNE-2023-00491},
pages = {618 - 634.e7},
year = {2017},
abstract = {Neural progenitor cells (NeuPCs) possess a unique nuclear
architecture that changes during differentiation.
Nucleoporins are linked with cell-type-specific gene
regulation, coupling physical changes in nuclear structure
to transcriptional output; but, whether and how they
coordinate with key fate-determining transcription factors
is unclear. Here we show that the nucleoporin Nup153
interacts with Sox2 in adult NeuPCs, where it is
indispensable for their maintenance and controls neuronal
differentiation. Genome-wide analyses show that Nup153 and
Sox2 bind and co-regulate hundreds of genes. Binding of
Nup153 to gene promoters or transcriptional end sites
correlates with increased or decreased gene expression,
respectively, and inhibiting Nup153 expression alters open
chromatin configurations at its target genes, disrupts
genomic localization of Sox2, and promotes differentiation
in vitro and a gliogenic fate switch in vivo. Together,
these findings reveal that nuclear structural proteins may
exert bimodal transcriptional effects to control cell fate.},
keywords = {Animals / Chromatin: metabolism / Gene Expression
Regulation / Genome / Mice / Neural Stem Cells: metabolism /
Neurogenesis: genetics / Nuclear Pore Complex Proteins:
metabolism / Protein Binding / SOXB1 Transcription Factors:
metabolism / Transcription, Genetic / Nup153 (Other) / Sox2
(Other) / adult neurogenesis (Other) / bimodal gene
regulation (Other) / cell fate (Other) / key transcription
factors (Other) / neural differentiation (Other) / neural
progenitor cells (Other) / nucleoporins (Other) / spatial
transcriptional regulation (Other) / Chromatin (NLM
Chemicals) / Nuclear Pore Complex Proteins (NLM Chemicals) /
Nup153 protein, mouse (NLM Chemicals) / SOXB1 Transcription
Factors (NLM Chemicals)},
ddc = {570},
pnm = {899 - ohne Topic (POF4-899)},
pid = {G:(DE-HGF)POF4-899},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:28919367},
doi = {10.1016/j.stem.2017.08.012},
url = {https://pub.dzne.de/record/257694},
}