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000257788 005__ 20240129114736.0
000257788 0247_ $$2doi$$a10.37188/lam.2022.023
000257788 037__ $$aDZNE-2023-00503
000257788 1001_ $$0P:(DE-HGF)0$$aKoukourakis, Nektarios$$b0
000257788 245__ $$aInvestigation of human organoid retina with digital holographic transmission matrix measurements
000257788 260__ $$c2022
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000257788 3367_ $$2BibTeX$$aARTICLE
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000257788 3367_ $$00$$2EndNote$$aJournal Article
000257788 500__ $$aJournal: Light: Advanced Manufacturing, ISSN: 2689-9620
000257788 520__ $$aAdvanced manufacturing of retinal organoid samples from human induced pluripotent stem cells represents a promising way to study the development of retinal diseases. The retina is an epithelium composed of different cell layers with unique optical properties and detects light by photoreceptor neurons for visual function. There are still many challenges in detecting early and distinct cellular changes in retinal disease. In this paper, we study the capability of the optical transmission matrix, which fully describes the transition of a light field propagating through a scattering sample. Despite its rich information content, the transmission matrix is commonly just used for light delivery through scattering media. Digital holography is employed to measure the complex light-field information of the transmitted light. We demonstrate that singular value decomposition of the transmission matrix allows to discriminate phantom tissues with varying scattering coefficient. We apply these findings to retinal organoid tissues. Application of the protonophore carbonyl cyanide m-chloro-phenylhydrazone (CCCP), a known inducer of retinal damage in animals, caused cell death and structural changes in human retinal organoids, which resulted in distinct changes in the transmission matrix. Our data indicate that the analysis of the transmission matrix can distinguish pathologic changes of the retina towards the development of imaging-based biomarkers.
000257788 536__ $$0G:(DE-HGF)POF4-352$$a352 - Disease Mechanisms (POF4-352)$$cPOF4-352$$fPOF IV$$x0
000257788 588__ $$aDataset connected to CrossRef, Journals: pub.dzne.de
000257788 7001_ $$0P:(DE-2719)2811441$$aWagner, Felix$$b1$$udzne
000257788 7001_ $$0P:(DE-HGF)0$$aRothe, Stefan$$b2
000257788 7001_ $$0P:(DE-2719)2000041$$aKarl, Mike Oliver$$b3$$udzne
000257788 7001_ $$0P:(DE-HGF)0$$aCzarske, Jürgen W$$b4
000257788 773__ $$a10.37188/lam.2022.023$$gVol. 3, no. 2, p. 1 -$$n2$$p211-225$$v3$$y2022
000257788 8564_ $$uhttps://www.light-am.com/article/doi/10.37188/lam.2022.023
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000257788 9101_ $$0I:(DE-588)1065079516$$6P:(DE-2719)2811441$$aDeutsches Zentrum für Neurodegenerative Erkrankungen$$b1$$kDZNE
000257788 9101_ $$0I:(DE-588)1065079516$$6P:(DE-2719)2000041$$aDeutsches Zentrum für Neurodegenerative Erkrankungen$$b3$$kDZNE
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000257788 9141_ $$y2022
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000257788 915__ $$0LIC:(DE-HGF)CCBY4$$2HGFVOC$$aCreative Commons Attribution CC BY 4.0
000257788 9201_ $$0I:(DE-2719)1710004$$kAG Karl$$lRetina Regeneration and Degeneration$$x0
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