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@ARTICLE{AngladaHuguet:258261,
author = {Anglada-Huguet, Marta and Endepols, Heike and Sydow, Astrid
and Hilgers, Ronja and Neumaier, Bernd and Drzezga,
Alexander and Kaniyappan, Senthilvelrajan and Mandelkow,
Eckhard and Mandelkow, Eva-Maria},
title = {{R}eversal of {T}au-{D}ependent {C}ognitive {D}ecay by
{B}locking {A}denosine {A}1 {R}eceptors: {C}omparison of
{T}ransgenic {M}ouse {M}odels with {D}ifferent {L}evels of
{T}auopathy.},
journal = {International journal of molecular sciences},
volume = {24},
number = {11},
issn = {1422-0067},
address = {Basel},
publisher = {Molecular Diversity Preservation International},
reportid = {DZNE-2023-00607},
pages = {9260},
year = {2023},
abstract = {The accumulation of tau is a hallmark of several
neurodegenerative diseases and is associated with neuronal
hypoactivity and presynaptic dysfunction. Oral
administration of the adenosine A1 receptor antagonist
rolofylline (KW-3902) has previously been shown to reverse
spatial memory deficits and to normalize the basic synaptic
transmission in a mouse line expressing full-length
pro-aggregant tau (TauΔK) at low levels, with late onset of
disease. However, the efficacy of treatment remained to be
explored for cases of more aggressive tauopathy. Using a
combination of behavioral assays, imaging with several
PET-tracers, and analysis of brain tissue, we compared the
curative reversal of tau pathology by blocking adenosine A1
receptors in three mouse models expressing different types
and levels of tau and tau mutants. We show through positron
emission tomography using the tracer [18F]CPFPX (a selective
A1 receptor ligand) that intravenous injection of
rolofylline effectively blocks A1 receptors in the brain.
Moreover, when administered to TauΔK mice, rolofylline can
reverse tau pathology and synaptic decay. The beneficial
effects are also observed in a line with more aggressive tau
pathology, expressing the amyloidogenic repeat domain of tau
(TauRDΔK) with higher aggregation propensity. Both models
develop a progressive tau pathology with missorting,
phosphorylation, accumulation of tau, loss of synapses, and
cognitive decline. TauRDΔK causes pronounced
neurofibrillary tangle assembly concomitant with neuronal
death, whereas TauΔK accumulates only to tau pretangles
without overt neuronal loss. A third model tested, the
rTg4510 line, has a high expression of mutant TauP301L and
hence a very aggressive phenotype starting at ~3 months of
age. This line failed to reverse pathology upon rolofylline
treatment, consistent with a higher accumulation of
tau-specific PET tracers and inflammation. In conclusion,
blocking adenosine A1 receptors by rolofylline can reverse
pathology if the pathological potential of tau remains below
a threshold value that depends on concentration and
aggregation propensity.},
keywords = {Mice / Animals / Mice, Transgenic / Receptor, Adenosine A1:
genetics / Receptor, Adenosine A1: metabolism / tau
Proteins: genetics / tau Proteins: metabolism / Hippocampus:
metabolism / Tauopathies: drug therapy / Tauopathies:
genetics / Tauopathies: metabolism / Cognition / Disease
Models, Animal / Alzheimer’s disease (Other) /
Alzheimer’s disease (Other) / Alzheimer’s disease
(Other) / Alzheimer’s disease (Other) / PET (Other) /
adenosine A1 receptor (Other) / behavior (Other) /
rolofylline (Other) / synapses (Other) / tau protein (Other)
/ Receptor, Adenosine A1 (NLM Chemicals) / tau Proteins (NLM
Chemicals)},
cin = {AG Mandelkow 2 ; AG Mandelkow 2 / AG Mandelkow 1 ; AG
Mandelkow 1 / AG Schneider / AG Boecker},
ddc = {540},
cid = {I:(DE-2719)1013015 / I:(DE-2719)1013014 /
I:(DE-2719)1011305 / I:(DE-2719)1011202},
pnm = {352 - Disease Mechanisms (POF4-352) / 353 - Clinical and
Health Care Research (POF4-353)},
pid = {G:(DE-HGF)POF4-352 / G:(DE-HGF)POF4-353},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:37298211},
pmc = {pmc:PMC10252552},
doi = {10.3390/ijms24119260},
url = {https://pub.dzne.de/record/258261},
}