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@MISC{Riemenschneider:258264,
      author       = {Riemenschneider, Henrick and Edbauer, Dieter},
      title        = {{D}ataset: {B}ulk neocortical {RNA} sequencing of r{NLS}8
                      mice (on mixed {C}57{BL}/6{J} × {C}3{H}/{H}e{J} background
                      ) +/- active immunization with {C}-terminal h{TDP}-43
                      antigens},
      publisher    = {Gene Expression Omnibus},
      reportid     = {DZNE-2023-00610},
      year         = {2023},
      abstract     = {This data set reports RNA sequencing results obtained from
                      whole cerebral cortices of NEFH-tTa/tetO-hTDP43∆NLS
                      transgenic (“rNLS8”) mice that were actively immunized
                      with C-terminal fragments of the human TDP-43 protein. The
                      widely used rNLS8 mouse model overexpresses cytoplasmically
                      mislocalized human TDP-43 in a doxycycline-inducible manner,
                      mimicking ALS/FTD-like CNS pathology and motor dysfunction
                      (Walker et al., 2015). The purpose of this study was to
                      evaluate the safety profile and therapeutic potential of
                      various human TDP-43 epitopes (up to approximately 40 amino
                      acids in length) which were used as antigens for active
                      immunization in a rapidly progressing mouse model of TDP-43
                      proteinopathy. To this end, we pre-screened 15 peptide
                      antigens, collectively spanning the entire TDP-43 protein
                      sequence, for immunogenicity and safety. Next, we repeatedly
                      immunized “rNLS8” mice with the most promising antigens
                      prior to transgene induction, and performed bulk RNA
                      sequencing of the neocortex, since this region is
                      considerably affected by TDP-43 pathology in both humans and
                      the mouse model. We asked whether active immunization with
                      two different peptide combinations leading to high antibody
                      titers would affect – i.e. prevent – transcriptional
                      alterations upon transgene induction. In summary, we provide
                      a bulk neocortical gene expression profile of actively
                      immunized (and mock-treated) ALS/FTD-resembling “rNLS8”
                      mice.},
      cin          = {AG Edbauer},
      cid          = {I:(DE-2719)1110004},
      pnm          = {352 - Disease Mechanisms (POF4-352)},
      pid          = {G:(DE-HGF)POF4-352},
      typ          = {PUB:(DE-HGF)32},
      url          = {https://pub.dzne.de/record/258264},
}