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@ARTICLE{Siddiqui:259000,
author = {Siddiqui, Tohid and Cosacak, Mehmet Ilyas and Popova,
Stanislava and Bhattarai, Prabesh and Yilmaz, Elanur and
Lee, Annie J and Min, Yuhao and Wang, Xue and Allen, Mariet
and İş, Özkan and Atasavum, Zeynep Tansu and
Rodriguez-Muela, Natalia and Vardarajan, Badri N and
Flaherty, Delaney and Teich, Andrew F and Santa-Maria,
Ismael and Freudenberg, Uwe and Werner, Carsten and Tosto,
Giuseppe and Mayeux, Richard and Ertekin-Taner, Nilüfer and
Kizil, Caghan},
title = {{N}erve growth factor receptor ({N}gfr) induces neurogenic
plasticity by suppressing reactive astroglial
{L}cn2/{S}lc22a17 signaling in {A}lzheimer's disease.},
journal = {npj regenerative medicine},
volume = {8},
number = {1},
issn = {2057-3995},
address = {[London]},
publisher = {Nature Publishing Group},
reportid = {DZNE-2023-00713},
pages = {33},
year = {2023},
abstract = {Neurogenesis, crucial for brain resilience, is reduced in
Alzheimer's disease (AD) that induces astroglial reactivity
at the expense of the pro-neurogenic potential, and
restoring neurogenesis could counteract neurodegenerative
pathology. However, the molecular mechanisms promoting
pro-neurogenic astroglial fate despite AD pathology are
unknown. In this study, we used APP/PS1dE9 mouse model and
induced Nerve growth factor receptor (Ngfr) expression in
the hippocampus. Ngfr, which promotes neurogenic fate of
astroglia during the amyloid pathology-induced
neuroregeneration in zebrafish brain, stimulated
proliferative and neurogenic outcomes. Histological analyses
of the changes in proliferation and neurogenesis,
single-cell transcriptomics, spatial proteomics, and
functional knockdown studies showed that the induced
expression of Ngfr reduced the reactive astrocyte marker
Lipocalin-2 (Lcn2), which we found was sufficient to reduce
neurogenesis in astroglia. Anti-neurogenic effects of Lcn2
was mediated by Slc22a17, blockage of which recapitulated
the pro-neurogenicity by Ngfr. Long-term Ngfr expression
reduced amyloid plaques and Tau phosphorylation. Postmortem
human AD hippocampi and 3D human astroglial cultures showed
elevated LCN2 levels correlate with reactive gliosis and
reduced neurogenesis. Comparing transcriptional changes in
mouse, zebrafish, and human AD brains for cell intrinsic
differential gene expression and weighted gene co-expression
networks revealed common altered downstream effectors of
NGFR signaling, such as PFKP, which can enhance
proliferation and neurogenesis in vitro when blocked. Our
study suggests that the reactive non-neurogenic astroglia in
AD can be coaxed to a pro-neurogenic fate and AD pathology
can be alleviated with Ngfr. We suggest that enhancing
pro-neurogenic astroglial fate may have therapeutic
ramifications in AD.},
cin = {AG Kizil / AG Rodriguez-Muela},
ddc = {610},
cid = {I:(DE-2719)1710007 / I:(DE-2719)1713001},
pnm = {352 - Disease Mechanisms (POF4-352)},
pid = {G:(DE-HGF)POF4-352},
typ = {PUB:(DE-HGF)16},
pmc = {pmc:PMC10333226},
pubmed = {pmid:37429840},
doi = {10.1038/s41536-023-00311-5},
url = {https://pub.dzne.de/record/259000},
}