TY  - JOUR
AU  - Chen, Fushun
AU  - Köhler, Melina
AU  - Cucun, Gokhan
AU  - Takamiya, Masanari
AU  - Kizil, Caghan
AU  - Cosacak, Mehmet Ilyas
AU  - Rastegar, Sepand
TI  - sox1a:eGFP transgenic line and single-cell transcriptomics reveal the origin of zebrafish intraspinal serotonergic neurons.
JO  - iScience
VL  - 26
IS  - 8
SN  - 2589-0042
CY  - St. Louis
PB  - Elsevier
M1  - DZNE-2023-00769
SP  - 107342
PY  - 2023
AB  - Sox transcription factors are crucial for vertebrate nervous system development. In zebrafish embryo, sox1 genes are expressed in neural progenitor cells and neurons of ventral spinal cord. Our recent study revealed that the loss of sox1a and sox1b function results in a significant decline of V2 subtype neurons (V2s). Using single-cell RNA sequencing, we analyzed the transcriptome of sox1a lineage progenitors and neurons in the zebrafish spinal cord at four time points during embryonic development, employing the Tg(sox1a:eGFP) line. In addition to previously characterized sox1a-expressing neurons, we discovered the expression of sox1a in late-developing intraspinal serotonergic neurons (ISNs). Developmental trajectory analysis suggests that ISNs arise from lateral floor plate (LFP) progenitor cells. Pharmacological inhibition of the Notch signaling pathway revealed its role in negatively regulating LFP progenitor cell differentiation into ISNs. Our findings highlight the zebrafish LFP as a progenitor domain for ISNs, alongside known Kolmer-Agduhr (KA) and V3 interneurons.
KW  - Cell biology (Other)
KW  - Neuroscience (Other)
KW  - Omics (Other)
KW  - Transcriptomics (Other)
LB  - PUB:(DE-HGF)16
C6  - pmid:37529101
C2  - pmc:PMC10387610
DO  - DOI:10.1016/j.isci.2023.107342
UR  - https://pub.dzne.de/record/259697
ER  -