TY  - CHAP
AU  - Scholz, Rebekka
AU  - Brösamle, Desirée
AU  - Yuan, Xidi
AU  - Neher, Jonas J
AU  - Beyer, Marc
TI  - Combined Analysis of mRNA Expression and Open Chromatin in Microglia.
VL  - 2713
CY  - New York, NY
PB  - Springer US
M1  - DZNE-2023-00833
SN  - 978-1-0716-3436-3 (print)
T2  - Methods in Molecular Biology
SP  - 543 - 571
PY  - 2024
AB  - The advance of single-cell RNA-sequencing technologies in the past years has enabled unprecedented insights into the complexity and heterogeneity of microglial cell states in the homeostatic and diseased brain. This includes rather complex proteomic, metabolomic, morphological, transcriptomic, and epigenetic adaptations to external stimuli and challenges resulting in a novel concept of core microglia properties and functions. To uncover the regulatory programs facilitating the rapid transcriptomic adaptation in response to changes in the local microenvironment, the accessibility of gene bodies and gene regulatory elements can be assessed. Here, we describe the application of a previously published method for simultaneous high-throughput ATAC and RNA expression with sequencing (SHARE-seq) on microglia nuclei isolated from frozen mouse brain tissue.
KW  - Animals
KW  - Mice
KW  - Chromatin: genetics
KW  - Microglia
KW  - Proteomics
KW  - RNA, Messenger: genetics
KW  - RNA
KW  - Bioinformatics (Other)
KW  - Chromatin accessibility (Other)
KW  - Epigenetics (Other)
KW  - Microglia (Other)
KW  - Multiomics (Other)
KW  - Single-nucleus sequencing (Other)
KW  - Transcriptomics (Other)
KW  - Chromatin (NLM Chemicals)
KW  - RNA, Messenger (NLM Chemicals)
KW  - RNA (NLM Chemicals)
LB  - PUB:(DE-HGF)7
C6  - pmid:37639146
DO  - DOI:10.1007/978-1-0716-3437-0_35
UR  - https://pub.dzne.de/record/263611
ER  -