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@INBOOK{Scholz:263611,
author = {Scholz, Rebekka and Brösamle, Desirée and Yuan, Xidi and
Neher, Jonas J and Beyer, Marc},
title = {{C}ombined {A}nalysis of m{RNA} {E}xpression and {O}pen
{C}hromatin in {M}icroglia.},
volume = {2713},
address = {New York, NY},
publisher = {Springer US},
reportid = {DZNE-2023-00833},
isbn = {978-1-0716-3436-3 (print)},
series = {Methods in Molecular Biology},
pages = {543 - 571},
year = {2024},
comment = {Tissue-Resident Macrophages / Mass, Elvira (Editor) ; New
York, NY : Springer US, 2024, Chapter 35 ; ISSN:
1064-3745=1940-6029 ; ISBN:
978-1-0716-3436-3=978-1-0716-3437-0 ;
doi:10.1007/978-1-0716-3437-0},
booktitle = {Tissue-Resident Macrophages / Mass,
Elvira (Editor) ; New York, NY :
Springer US, 2024, Chapter 35 ; ISSN:
1064-3745=1940-6029 ; ISBN:
978-1-0716-3436-3=978-1-0716-3437-0 ;
doi:10.1007/978-1-0716-3437-0},
abstract = {The advance of single-cell RNA-sequencing technologies in
the past years has enabled unprecedented insights into the
complexity and heterogeneity of microglial cell states in
the homeostatic and diseased brain. This includes rather
complex proteomic, metabolomic, morphological,
transcriptomic, and epigenetic adaptations to external
stimuli and challenges resulting in a novel concept of core
microglia properties and functions. To uncover the
regulatory programs facilitating the rapid transcriptomic
adaptation in response to changes in the local
microenvironment, the accessibility of gene bodies and gene
regulatory elements can be assessed. Here, we describe the
application of a previously published method for
simultaneous high-throughput ATAC and RNA expression with
sequencing (SHARE-seq) on microglia nuclei isolated from
frozen mouse brain tissue.},
keywords = {Animals / Mice / Chromatin: genetics / Microglia /
Proteomics / RNA, Messenger: genetics / RNA / Bioinformatics
(Other) / Chromatin accessibility (Other) / Epigenetics
(Other) / Microglia (Other) / Multiomics (Other) /
Single-nucleus sequencing (Other) / Transcriptomics (Other)
/ Chromatin (NLM Chemicals) / RNA, Messenger (NLM Chemicals)
/ RNA (NLM Chemicals)},
cin = {AG Beyer / AG Neher (Tübingen) / PRECISE},
ddc = {570},
cid = {I:(DE-2719)1013035 / I:(DE-2719)1210012 /
I:(DE-2719)1013031},
pnm = {351 - Brain Function (POF4-351) / 352 - Disease Mechanisms
(POF4-352)},
pid = {G:(DE-HGF)POF4-351 / G:(DE-HGF)POF4-352},
experiment = {EXP:(DE-2719)PRECISE-20190321},
typ = {PUB:(DE-HGF)7},
pubmed = {pmid:37639146},
doi = {10.1007/978-1-0716-3437-0_35},
url = {https://pub.dzne.de/record/263611},
}