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@ARTICLE{Michanski:263802,
      author       = {Michanski, Susann and Kapoor, Rohan and Steyer, Anna M and
                      Möbius, Wiebke and Früholz, Iris and Ackermann, Frauke and
                      Gültas, Mehmet and Garner, Craig C and Hamra, F Kent and
                      Neef, Jakob and Strenzke, Nicola and Moser, Tobias and
                      Wichmann, Carolin},
      title        = {{P}iccolino is required for ribbon architecture at cochlear
                      inner hair cell synapses and for hearing.},
      journal      = {EMBO reports},
      volume       = {24},
      number       = {9},
      issn         = {1469-221X},
      address      = {Hoboken, NJ [u.a.]},
      publisher    = {Wiley},
      reportid     = {DZNE-2023-00888},
      pages        = {e56702},
      year         = {2023},
      abstract     = {Cochlear inner hair cells (IHCs) form specialized ribbon
                      synapses with spiral ganglion neurons that tirelessly
                      transmit sound information at high rates over long time
                      periods with extreme temporal precision. This functional
                      specialization is essential for sound encoding and is
                      attributed to a distinct molecular machinery with unique
                      players or splice variants compared to conventional neuronal
                      synapses. Among these is the active zone (AZ) scaffold
                      protein piccolo/aczonin, which is represented by its short
                      splice variant piccolino at cochlear and retinal ribbon
                      synapses. While the function of piccolo at synapses of the
                      central nervous system has been intensively investigated,
                      the role of piccolino at IHC synapses remains unclear. In
                      this study, we characterize the structure and function of
                      IHC synapses in piccolo gene-trap mutant rats (Pclogt/gt ).
                      We find a mild hearing deficit with elevated thresholds and
                      reduced amplitudes of auditory brainstem responses. Ca2+
                      channel distribution and ribbon morphology are altered in
                      apical IHCs, while their presynaptic function seems to be
                      unchanged. We conclude that piccolino contributes to the AZ
                      organization in IHCs and is essential for normal hearing.},
      keywords     = {Rats / Animals / Hair Cells, Auditory, Inner / Hearing:
                      physiology / Synapses: physiology / Cochlea / Spiral
                      Ganglion: metabolism / Cytoskeletal Proteins: metabolism /
                      Neuropeptides / Ca2+ channel (Other) / active zone (Other) /
                      cochlea (Other) / hearing (Other) / synaptic vesicle (Other)
                      / Pclo protein, rat (NLM Chemicals) / Cytoskeletal Proteins
                      (NLM Chemicals) / Neuropeptides (NLM Chemicals)},
      cin          = {AG Ackermann / AG Garner},
      ddc          = {570},
      cid          = {I:(DE-2719)1813004 / I:(DE-2719)1810001},
      pnm          = {351 - Brain Function (POF4-351)},
      pid          = {G:(DE-HGF)POF4-351},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:37477166},
      pmc          = {pmc:PMC10481675},
      doi          = {10.15252/embr.202256702},
      url          = {https://pub.dzne.de/record/263802},
}