000265755 001__ 265755
000265755 005__ 20231120155400.0
000265755 0247_ $$2doi$$a10.3389/fncel.2023.1166641
000265755 0247_ $$2pmid$$apmid:37868194
000265755 0247_ $$2pmc$$apmc:PMC10587494
000265755 0247_ $$2altmetric$$aaltmetric:155261234
000265755 037__ $$aDZNE-2023-01030
000265755 041__ $$aEnglish
000265755 082__ $$a610
000265755 1001_ $$aCarido, Madalena$$b0
000265755 245__ $$aReliability of human retina organoid generation from hiPSC-derived neuroepithelial cysts.
000265755 260__ $$aLausanne$$bFrontiers Research Foundation$$c2023
000265755 3367_ $$2DRIVER$$aarticle
000265755 3367_ $$2DataCite$$aOutput Types/Journal article
000265755 3367_ $$0PUB:(DE-HGF)16$$2PUB:(DE-HGF)$$aJournal Article$$bjournal$$mjournal$$s1700062829_31974
000265755 3367_ $$2BibTeX$$aARTICLE
000265755 3367_ $$2ORCID$$aJOURNAL_ARTICLE
000265755 3367_ $$00$$2EndNote$$aJournal Article
000265755 520__ $$aThe possible applications for human retinal organoids (HROs) derived from human induced pluripotent stem cells (hiPSC) rely on the robustness and transferability of the methodology for their generation. Standardized strategies and parameters to effectively assess, compare, and optimize organoid protocols are starting to be established, but are not yet complete. To advance this, we explored the efficiency and reliability of a differentiation method, called CYST protocol, that facilitates retina generation by forming neuroepithelial cysts from hiPSC clusters. Here, we tested seven different hiPSC lines which reproducibly generated HROs. Histological and ultrastructural analyses indicate that HRO differentiation and maturation are regulated. The different hiPSC lines appeared to be a larger source of variance than experimental rounds. Although previous reports have shown that HROs in several other protocols contain a rather low number of cones, HROs from the CYST protocol are consistently richer in cones and with a comparable ratio of cones, rods, and Müller glia. To provide further insight into HRO cell composition, we studied single cell RNA sequencing data and applied CaSTLe, a transfer learning approach. Additionally, we devised a potential strategy to systematically evaluate different organoid protocols side-by-side through parallel differentiation from the same hiPSC batches: In an explorative study, the CYST protocol was compared to a conceptually different protocol based on the formation of cell aggregates from single hiPSCs. Comparing four hiPSC lines showed that both protocols reproduced key characteristics of retinal epithelial structure and cell composition, but the CYST protocol provided a higher HRO yield. So far, our data suggest that CYST-derived HROs remained stable up to at least day 200, while single hiPSC-derived HROs showed spontaneous pathologic changes by day 200. Overall, our data provide insights into the efficiency, reproducibility, and stability of the CYST protocol for generating HROs, which will be useful for further optimizing organoid systems, as well as for basic and translational research applications.
000265755 536__ $$0G:(DE-HGF)POF4-352$$a352 - Disease Mechanisms (POF4-352)$$cPOF4-352$$fPOF IV$$x0
000265755 588__ $$aDataset connected to CrossRef, PubMed, , Journals: pub.dzne.de
000265755 650_7 $$2Other$$agliosis
000265755 650_7 $$2Other$$ahiPSC
000265755 650_7 $$2Other$$ahuman
000265755 650_7 $$2Other$$aneurodevelopment
000265755 650_7 $$2Other$$aorganoid
000265755 650_7 $$2Other$$apathology
000265755 650_7 $$2Other$$aretina
000265755 650_7 $$2Other$$astem cells
000265755 7001_ $$0P:(DE-2719)2810390$$aVölkner, Manuela$$b1$$eFirst author$$udzne
000265755 7001_ $$aSteinheuer, Lisa Maria$$b2
000265755 7001_ $$0P:(DE-2719)2811441$$aWagner, Felix$$b3$$udzne
000265755 7001_ $$aKurth, Thomas$$b4
000265755 7001_ $$0P:(DE-2719)9001836$$aDumler, Natalie$$b5$$udzne
000265755 7001_ $$0P:(DE-2719)9001420$$aUlusoy, Selen$$b6$$udzne
000265755 7001_ $$0P:(DE-2719)2812103$$aWieneke, Stephanie$$b7
000265755 7001_ $$aNorniella, Anabel Villanueva$$b8
000265755 7001_ $$0P:(DE-2719)2810498$$aGolfieri, Cristina$$b9
000265755 7001_ $$aKhattak, Shahryar$$b10
000265755 7001_ $$0P:(DE-2719)9001669$$aSchoenfelder, Bruno$$b11$$udzne
000265755 7001_ $$0P:(DE-2719)9002606$$aScamozzi, Maria$$b12$$udzne
000265755 7001_ $$0P:(DE-2719)2810521$$aZoschke, Katja$$b13$$udzne
000265755 7001_ $$aCanzler, Sebastian$$b14
000265755 7001_ $$aHackermüller, Jörg$$b15
000265755 7001_ $$aAder, Marius$$b16
000265755 7001_ $$0P:(DE-2719)2000041$$aKarl, Mike O$$b17$$eLast author$$udzne
000265755 773__ $$0PERI:(DE-600)2452963-1$$a10.3389/fncel.2023.1166641$$gVol. 17, p. 1166641$$p1166641$$tFrontiers in cellular neuroscience$$v17$$x1662-5102$$y2023
000265755 8564_ $$uhttps://pub.dzne.de/record/265755/files/DZNE-2023-01030.pdf$$yOpenAccess
000265755 8564_ $$uhttps://pub.dzne.de/record/265755/files/DZNE-2023-01030.pdf?subformat=pdfa$$xpdfa$$yOpenAccess
000265755 909CO $$ooai:pub.dzne.de:265755$$pdnbdelivery$$pdriver$$pVDB$$popen_access$$popenaire
000265755 9101_ $$0I:(DE-588)1065079516$$6P:(DE-2719)2810390$$aDeutsches Zentrum für Neurodegenerative Erkrankungen$$b1$$kDZNE
000265755 9101_ $$0I:(DE-HGF)0$$6P:(DE-2719)2811441$$aExternal Institute$$b3$$kExtern
000265755 9101_ $$0I:(DE-HGF)0$$6P:(DE-2719)9001836$$aExternal Institute$$b5$$kExtern
000265755 9101_ $$0I:(DE-HGF)0$$6P:(DE-2719)9001420$$aExternal Institute$$b6$$kExtern
000265755 9101_ $$0I:(DE-588)1065079516$$6P:(DE-2719)2812103$$aDeutsches Zentrum für Neurodegenerative Erkrankungen$$b7$$kDZNE
000265755 9101_ $$0I:(DE-588)1065079516$$6P:(DE-2719)2810498$$aDeutsches Zentrum für Neurodegenerative Erkrankungen$$b9$$kDZNE
000265755 9101_ $$0I:(DE-588)1065079516$$6P:(DE-2719)9001669$$aDeutsches Zentrum für Neurodegenerative Erkrankungen$$b11$$kDZNE
000265755 9101_ $$0I:(DE-HGF)0$$6P:(DE-2719)9002606$$aExternal Institute$$b12$$kExtern
000265755 9101_ $$0I:(DE-588)1065079516$$6P:(DE-2719)2810521$$aDeutsches Zentrum für Neurodegenerative Erkrankungen$$b13$$kDZNE
000265755 9101_ $$0I:(DE-588)1065079516$$6P:(DE-2719)2000041$$aDeutsches Zentrum für Neurodegenerative Erkrankungen$$b17$$kDZNE
000265755 9131_ $$0G:(DE-HGF)POF4-352$$1G:(DE-HGF)POF4-350$$2G:(DE-HGF)POF4-300$$3G:(DE-HGF)POF4$$4G:(DE-HGF)POF$$aDE-HGF$$bGesundheit$$lNeurodegenerative Diseases$$vDisease Mechanisms$$x0
000265755 9141_ $$y2023
000265755 915__ $$0StatID:(DE-HGF)0160$$2StatID$$aDBCoverage$$bEssential Science Indicators$$d2023-08-29
000265755 915__ $$0StatID:(DE-HGF)1190$$2StatID$$aDBCoverage$$bBiological Abstracts$$d2023-08-29
000265755 915__ $$0LIC:(DE-HGF)CCBY4$$2HGFVOC$$aCreative Commons Attribution CC BY 4.0
000265755 915__ $$0StatID:(DE-HGF)0501$$2StatID$$aDBCoverage$$bDOAJ Seal$$d2021-05-13T10:30:24Z
000265755 915__ $$0StatID:(DE-HGF)0500$$2StatID$$aDBCoverage$$bDOAJ$$d2021-05-13T10:30:24Z
000265755 915__ $$0StatID:(DE-HGF)0113$$2StatID$$aWoS$$bScience Citation Index Expanded$$d2023-08-29
000265755 915__ $$0StatID:(DE-HGF)0700$$2StatID$$aFees$$d2023-08-29
000265755 915__ $$0StatID:(DE-HGF)0510$$2StatID$$aOpenAccess
000265755 915__ $$0StatID:(DE-HGF)0030$$2StatID$$aPeer Review$$bDOAJ : Anonymous peer review$$d2021-05-13T10:30:24Z
000265755 915__ $$0StatID:(DE-HGF)0561$$2StatID$$aArticle Processing Charges$$d2023-08-29
000265755 915__ $$0StatID:(DE-HGF)0100$$2StatID$$aJCR$$bFRONT CELL NEUROSCI : 2022$$d2023-10-25
000265755 915__ $$0StatID:(DE-HGF)0200$$2StatID$$aDBCoverage$$bSCOPUS$$d2023-10-25
000265755 915__ $$0StatID:(DE-HGF)0300$$2StatID$$aDBCoverage$$bMedline$$d2023-10-25
000265755 915__ $$0StatID:(DE-HGF)0320$$2StatID$$aDBCoverage$$bPubMed Central$$d2023-10-25
000265755 915__ $$0StatID:(DE-HGF)0199$$2StatID$$aDBCoverage$$bClarivate Analytics Master Journal List$$d2023-10-25
000265755 915__ $$0StatID:(DE-HGF)1050$$2StatID$$aDBCoverage$$bBIOSIS Previews$$d2023-10-25
000265755 915__ $$0StatID:(DE-HGF)0150$$2StatID$$aDBCoverage$$bWeb of Science Core Collection$$d2023-10-25
000265755 915__ $$0StatID:(DE-HGF)1040$$2StatID$$aDBCoverage$$bZoological Record$$d2023-10-25
000265755 915__ $$0StatID:(DE-HGF)9905$$2StatID$$aIF >= 5$$bFRONT CELL NEUROSCI : 2022$$d2023-10-25
000265755 9201_ $$0I:(DE-2719)1710004$$kAG Karl$$lRetina Regeneration and Degeneration$$x0
000265755 980__ $$ajournal
000265755 980__ $$aVDB
000265755 980__ $$aUNRESTRICTED
000265755 980__ $$aI:(DE-2719)1710004
000265755 9801_ $$aFullTexts