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@ARTICLE{LechadoTerradas:266473,
author = {Lechado-Terradas, Anna and Schepers, Sandra and Zittlau,
Katharina I. and Sharma, Karan and Ok, Orkun and Fitzgerald,
Julia C. and Geimer, Stefan and Westermann, Benedikt and
Macek, Boris and Kahle, Philipp},
title = {{P}arkin-dependent mitophagy occurs via
proteasome-dependent steps sequentially targeting separate
mitochondrial sub-compartments for autophagy},
journal = {Autophagy reports},
volume = {1},
number = {1},
issn = {2769-4127},
address = {London},
publisher = {Taylor $\&$ Francis Group},
reportid = {DZNE-2023-01158},
pages = {576 - 602},
year = {2022},
abstract = {PINK1/parkin-dependent mitophagy initially involves
(phospho)ubiquitin-directed proteasome-dependent degradation
of certain outer mitochondrial membrane (OMM) proteins (e.g.
mitofusins) and the recruitment of autophagy adaptors to a
group of ubiquitinated OMM proteins, eventually leading to
autophagic removal of damaged mitochondria in stressed
cells. Here we provide evidence that mitochondrial
degradation occurs via stepwise delivery of separate
mitochondrial sub-compartments for autophagic degradation.
OMM and inner mitochondrial material appears to become
separately isolated for autophagolysosomal degradation, not
only in parkin-overexpressing HeLa cells but also in cells
that express endogenous parkin (human embryonic kidney cells
and neural progenitor cells) with slower mitophagy kinetics.
The remaining inner mitochondrial material becomes degraded
only after much prolonged membrane depolarization,
potentially involving another proteasome-sensitive step. The
present combined microscopy and proteomics analyses support
the idea that cell stress-induced parkin-dependent mitophagy
is a complex multi-step process with distinct mitochondrial
sub-compartments being separately targeted for autophagic
degradation.},
cin = {AG Kahle},
ddc = {610},
cid = {I:(DE-2719)1210000-4},
pnm = {352 - Disease Mechanisms (POF4-352)},
pid = {G:(DE-HGF)POF4-352},
typ = {PUB:(DE-HGF)16},
doi = {10.1080/27694127.2022.2143214},
url = {https://pub.dzne.de/record/266473},
}