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@ARTICLE{Passos:267514,
author = {Passos, Vania and Henkel, Lisa M and Wang, Jiayi and
Zapatero-Belinchón, Francisco J and Möller, Rebecca and
Sun, Guorong and Waltl, Inken and Schneider, Talia and
Wachs, Amelie and Ritter, Birgit and Kropp, Kai A and Zhu,
Shuyong and Deleidi, Michela and Kalinke, Ulrich and Schulz,
Thomas F and Höglinger, Günter and Gerold, Gisa and
Wegner, Florian and Viejo-Borbolla, Abel},
title = {{I}nnate immune response to {SARS}-{C}o{V}-2 infection
contributes to neuronal damage in human i{PSC}-derived
peripheral neurons.},
journal = {Journal of medical virology},
volume = {96},
number = {2},
issn = {0146-6615},
address = {Bognor Regis [u.a.]},
publisher = {Wiley},
reportid = {DZNE-2024-00160},
pages = {e29455},
year = {2024},
abstract = {Severe acute respiratory coronavirus 2 (SARS-CoV-2) causes
neurological disease in the peripheral and central nervous
system (PNS and CNS, respectively) of some patients. It is
not clear whether SARS-CoV-2 infection or the subsequent
immune response are the key factors that cause neurological
disease. Here, we addressed this question by infecting human
induced pluripotent stem cell-derived CNS and PNS neurons
with SARS-CoV-2. SARS-CoV-2 infected a low number of CNS
neurons and did not elicit a robust innate immune response.
On the contrary, SARS-CoV-2 infected a higher number of PNS
neurons. This resulted in expression of interferon (IFN)
λ1, several IFN-stimulated genes and proinflammatory
cytokines. The PNS neurons also displayed alterations
characteristic of neuronal damage, as increased levels of
sterile alpha and Toll/interleukin receptor motif-containing
protein 1, amyloid precursor protein and α-synuclein, and
lower levels of cytoskeletal proteins. Interestingly,
blockade of the Janus kinase and signal transducer and
activator of transcription pathway by Ruxolitinib did not
increase SARS-CoV-2 infection, but reduced neuronal damage,
suggesting that an exacerbated neuronal innate immune
response contributes to pathogenesis in the PNS. Our results
provide a basis to study coronavirus disease 2019 (COVID-19)
related neuronal pathology and to test future preventive or
therapeutic strategies.},
keywords = {Humans / COVID-19 / Induced Pluripotent Stem Cells /
SARS-CoV-2 / Immunity, Innate / Neurons / JAK/STAT (Other) /
SARM1 (Other) / SARS-CoV-2 (Other) / iPSC-derived peripheral
neurons (Other) / interferon (Other) / neuronal damage
(Other)},
cin = {AG Deleidi},
ddc = {610},
cid = {I:(DE-2719)1210011},
pnm = {352 - Disease Mechanisms (POF4-352)},
pid = {G:(DE-HGF)POF4-352},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:38323709},
doi = {10.1002/jmv.29455},
url = {https://pub.dzne.de/record/267514},
}