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000268500 1001_ $$00000-0003-4845-2228$$aShrouder, Joshua James$$b0
000268500 245__ $$aContinued dysfunction of capillary pericytes promotes no-reflow after experimental stroke in vivo.
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000268500 520__ $$aIncomplete reperfusion of the microvasculature ('no-reflow') after ischaemic stroke damages salvageable brain tissue. Previous ex vivo studies suggest pericytes are vulnerable to ischaemia and may exacerbate no-reflow, but the viability of pericytes and their association with no-reflow remains under-explored in vivo. Using longitudinal in vivo two-photon single-cell imaging over 7 days, we showed that 87% of pericytes constrict during cerebral ischaemia and remain constricted post reperfusion, and 50% of the pericyte population are acutely damaged. Moreover, we revealed ischaemic pericytes to be fundamentally implicated in capillary no-reflow by limiting and arresting blood flow within the first 24 h post stroke. Despite sustaining acute membrane damage, we observed that over half of all cortical pericytes survived ischaemia and responded to vasoactive stimuli, upregulated unique transcriptomic profiles and replicated. Finally, we demonstrated the delayed recovery of capillary diameter by ischaemic pericytes after reperfusion predicted vessel reconstriction in the subacute phase of stroke. Cumulatively, these findings demonstrate that surviving cortical pericytes remain both viable and promising therapeutic targets to counteract no-reflow after ischaemic stroke.
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000268500 650_7 $$2Other$$acerebral ischaemia
000268500 650_7 $$2Other$$aischaemic stroke
000268500 650_7 $$2Other$$ano-reflow
000268500 650_7 $$2Other$$apericytes
000268500 650_7 $$2Other$$areperfusion
000268500 650_2 $$2MeSH$$aHumans
000268500 650_2 $$2MeSH$$aStroke
000268500 650_2 $$2MeSH$$aPericytes: physiology
000268500 650_2 $$2MeSH$$aBrain Ischemia
000268500 650_2 $$2MeSH$$aIschemic Stroke
000268500 650_2 $$2MeSH$$aCerebral Infarction
000268500 7001_ $$0P:(DE-2719)9001033$$aCalandra, Gian-Marco$$b1$$udzne
000268500 7001_ $$0P:(DE-2719)2810523$$aFilser, Severin$$b2$$udzne
000268500 7001_ $$aVarga, Daniel Peter$$b3
000268500 7001_ $$aBesson-Girard, Simon$$b4
000268500 7001_ $$aMamrak, Uta$$b5
000268500 7001_ $$aDorok, Maximilian$$b6
000268500 7001_ $$aBulut-Impraim, Buket$$b7
000268500 7001_ $$aSeker, Fatma Burcu$$b8
000268500 7001_ $$0P:(DE-2719)9001112$$aGesierich, Benno$$b9$$udzne
000268500 7001_ $$aLaredo, Fabio$$b10
000268500 7001_ $$aWehn, Antonia Clarissa$$b11
000268500 7001_ $$aKhalin, Igor$$b12
000268500 7001_ $$aBayer, Patrick$$b13
000268500 7001_ $$00000-0002-9069-2594$$aLiesz, Arthur$$b14
000268500 7001_ $$0P:(DE-2719)9002754$$aGökce, Ozgun$$b15$$udzne
000268500 7001_ $$0P:(DE-2719)9000853$$aPlesnila, Nikolaus$$b16
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