TY  - JOUR
AU  - Zhang, Yun
AU  - Sung, Hsin-Ho
AU  - Ziegler, Anna
AU  - Wu, Ying-Chieh
AU  - Viais, Ricardo
AU  - Sánchez-Huertas, Carlos
AU  - Kilo, Lukas
AU  - Agircan, Fikret Gurkan
AU  - Cheng, Ying-Ju
AU  - Mouri, Kousuke
AU  - Uemura, Tadashi
AU  - Lüders, Jens
AU  - Chien, Cheng-Ting
AU  - Tavosanis, Gaia
TI  - Augmin complex activity finetunes dendrite morphology through non-centrosomal microtubule nucleation in vivo.
JO  - Journal of cell science
VL  - 137
IS  - 9
SN  - 0370-2952
CY  - Cambridge
PB  - Company of Biologists Limited
M1  - DZNE-2024-00571
SP  - jcs261512
PY  - 2024
AB  - During development, neurons achieve a stereotyped neuron type-specific morphology, which relies on dynamic support by microtubules (MTs). An important player is the augmin complex (hereafter augmin), which binds to existing MT filaments and recruits the γ-tubulin ring complex (γ-TuRC), to form branched MTs. In cultured neurons, augmin is important for neurite formation. However, little is known about the role of augmin during neurite formation in vivo. Here, we have revisited the role of mammalian augmin in culture and then turned towards the class four Drosophila dendritic arborization (c4da) neurons. We show that MT density is maintained through augmin in cooperation with the γ-TuRC in vivo. Mutant c4da neurons show a reduction of newly emerging higher-order dendritic branches and in turn also a reduced number of their characteristic space-filling higher-order branchlets. Taken together, our data reveal a cooperative function for augmin with the γ-TuRC in forming enough MTs needed for the appropriate differentiation of morphologically complex dendrites in vivo.
KW  - Animals
KW  - Microtubules: metabolism
KW  - Dendrites: metabolism
KW  - Drosophila Proteins: metabolism
KW  - Drosophila Proteins: genetics
KW  - Microtubule-Associated Proteins: metabolism
KW  - Microtubule-Associated Proteins: genetics
KW  - Drosophila melanogaster: metabolism
KW  - Tubulin: metabolism
KW  - Drosophila: metabolism
KW  - Humans
KW  - Neurons: metabolism
KW  - Neurons: cytology
KW  - γ-tubulin (Other)
KW  - Drosophila c4da neurons (Other)
KW  - Augmin (Other)
KW  - HAUS (Other)
KW  - Hippocampal neurons (Other)
KW  - Microtubules (Other)
KW  - Neuronal dendrites (Other)
KW  - γ-tubulin (Other)
KW  - Drosophila Proteins (NLM Chemicals)
KW  - Microtubule-Associated Proteins (NLM Chemicals)
KW  - Tubulin (NLM Chemicals)
LB  - PUB:(DE-HGF)16
C2  - pmc:PMC11128282
C6  - pmid:38587100
DO  - DOI:10.1242/jcs.261512
UR  - https://pub.dzne.de/record/269530
ER  -