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@ARTICLE{Zhang:269530,
author = {Zhang, Yun and Sung, Hsin-Ho and Ziegler, Anna and Wu,
Ying-Chieh and Viais, Ricardo and Sánchez-Huertas, Carlos
and Kilo, Lukas and Agircan, Fikret Gurkan and Cheng,
Ying-Ju and Mouri, Kousuke and Uemura, Tadashi and Lüders,
Jens and Chien, Cheng-Ting and Tavosanis, Gaia},
title = {{A}ugmin complex activity finetunes dendrite morphology
through non-centrosomal microtubule nucleation in vivo.},
journal = {Journal of cell science},
volume = {137},
number = {9},
issn = {0370-2952},
address = {Cambridge},
publisher = {Company of Biologists Limited},
reportid = {DZNE-2024-00571},
pages = {jcs261512},
year = {2024},
abstract = {During development, neurons achieve a stereotyped neuron
type-specific morphology, which relies on dynamic support by
microtubules (MTs). An important player is the augmin
complex (hereafter augmin), which binds to existing MT
filaments and recruits the γ-tubulin ring complex
(γ-TuRC), to form branched MTs. In cultured neurons, augmin
is important for neurite formation. However, little is known
about the role of augmin during neurite formation in vivo.
Here, we have revisited the role of mammalian augmin in
culture and then turned towards the class four Drosophila
dendritic arborization (c4da) neurons. We show that MT
density is maintained through augmin in cooperation with the
γ-TuRC in vivo. Mutant c4da neurons show a reduction of
newly emerging higher-order dendritic branches and in turn
also a reduced number of their characteristic space-filling
higher-order branchlets. Taken together, our data reveal a
cooperative function for augmin with the γ-TuRC in forming
enough MTs needed for the appropriate differentiation of
morphologically complex dendrites in vivo.},
keywords = {Animals / Microtubules: metabolism / Dendrites: metabolism
/ Drosophila Proteins: metabolism / Drosophila Proteins:
genetics / Microtubule-Associated Proteins: metabolism /
Microtubule-Associated Proteins: genetics / Drosophila
melanogaster: metabolism / Tubulin: metabolism / Drosophila:
metabolism / Humans / Neurons: metabolism / Neurons:
cytology / γ-tubulin (Other) / Drosophila c4da neurons
(Other) / Augmin (Other) / HAUS (Other) / Hippocampal
neurons (Other) / Microtubules (Other) / Neuronal dendrites
(Other) / γ-tubulin (Other) / Drosophila Proteins (NLM
Chemicals) / Microtubule-Associated Proteins (NLM Chemicals)
/ Tubulin (NLM Chemicals)},
cin = {AG Tavosanis},
ddc = {570},
cid = {I:(DE-2719)1013018},
pnm = {351 - Brain Function (POF4-351)},
pid = {G:(DE-HGF)POF4-351},
typ = {PUB:(DE-HGF)16},
pmc = {pmc:PMC11128282},
pubmed = {pmid:38587100},
doi = {10.1242/jcs.261512},
url = {https://pub.dzne.de/record/269530},
}