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000270295 1001_ $$aGroschup, Bernhard$$b0
000270295 245__ $$aProbing intracellular potassium dynamics in neurons with the genetically encoded sensor lc-LysM GEPII 1.0 in vitro and in vivo.
000270295 260__ $$a[London]$$bMacmillan Publishers Limited, part of Springer Nature$$c2024
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000270295 520__ $$aNeuronal activity is accompanied by a net outflow of potassium ions (K+) from the intra- to the extracellular space. While extracellular [K+] changes during neuronal activity are well characterized, intracellular dynamics have been less well investigated due to lack of respective probes. In the current study we characterized the FRET-based K+ biosensor lc-LysM GEPII 1.0 for its capacity to measure intracellular [K+] changes in primary cultured neurons and in mouse cortical neurons in vivo. We found that lc-LysM GEPII 1.0 can resolve neuronal [K+] decreases in vitro during seizure-like and intense optogenetically evoked activity. [K+] changes during single action potentials could not be recorded. We confirmed these findings in vivo by expressing lc-LysM GEPII 1.0 in mouse cortical neurons and performing 2-photon fluorescence lifetime imaging. We observed an increase in the fluorescence lifetime of lc-LysM GEPII 1.0 during periinfarct depolarizations, which indicates a decrease in intracellular neuronal [K+]. Our findings suggest that lc-LysM GEPII 1.0 can be used to measure large changes in [K+] in neurons in vitro and in vivo but requires optimization to resolve smaller changes as observed during single action potentials.
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000270295 650_7 $$0RWP5GA015D$$2NLM Chemicals$$aPotassium
000270295 650_2 $$2MeSH$$aAnimals
000270295 650_2 $$2MeSH$$aPotassium: metabolism
000270295 650_2 $$2MeSH$$aNeurons: metabolism
000270295 650_2 $$2MeSH$$aMice
000270295 650_2 $$2MeSH$$aBiosensing Techniques: methods
000270295 650_2 $$2MeSH$$aAction Potentials
000270295 650_2 $$2MeSH$$aCells, Cultured
000270295 650_2 $$2MeSH$$aFluorescence Resonance Energy Transfer: methods
000270295 650_2 $$2MeSH$$aOptogenetics: methods
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000270295 7001_ $$0P:(DE-2719)9001033$$aCalandra, Gian-Marco$$b1$$udzne
000270295 7001_ $$aRaitmayr, Constanze$$b2
000270295 7001_ $$aShrouder, Joshua$$b3
000270295 7001_ $$aLlovera, Gemma$$b4
000270295 7001_ $$aZaki, Asal Ghaffari$$b5
000270295 7001_ $$aBurgstaller, Sandra$$b6
000270295 7001_ $$aBischof, Helmut$$b7
000270295 7001_ $$aEroglu, Emrah$$b8
000270295 7001_ $$aLiesz, Arthur$$b9
000270295 7001_ $$aMalli, Roland$$b10
000270295 7001_ $$0P:(DE-2719)2810523$$aFilser, Severin$$b11$$eLast author$$udzne
000270295 7001_ $$aPlesnila, Nikolaus$$b12
000270295 773__ $$0PERI:(DE-600)2615211-3$$a10.1038/s41598-024-62993-1$$gVol. 14, no. 1, p. 13753$$n1$$p13753$$tScientific reports$$v14$$x2045-2322$$y2024
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