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@ARTICLE{Groschup:270295,
      author       = {Groschup, Bernhard and Calandra, Gian-Marco and Raitmayr,
                      Constanze and Shrouder, Joshua and Llovera, Gemma and Zaki,
                      Asal Ghaffari and Burgstaller, Sandra and Bischof, Helmut
                      and Eroglu, Emrah and Liesz, Arthur and Malli, Roland and
                      Filser, Severin and Plesnila, Nikolaus},
      title        = {{P}robing intracellular potassium dynamics in neurons with
                      the genetically encoded sensor lc-{L}ys{M} {GEPII} 1.0 in
                      vitro and in vivo.},
      journal      = {Scientific reports},
      volume       = {14},
      number       = {1},
      issn         = {2045-2322},
      address      = {[London]},
      publisher    = {Macmillan Publishers Limited, part of Springer Nature},
      reportid     = {DZNE-2024-00767},
      pages        = {13753},
      year         = {2024},
      abstract     = {Neuronal activity is accompanied by a net outflow of
                      potassium ions (K+) from the intra- to the extracellular
                      space. While extracellular [K+] changes during neuronal
                      activity are well characterized, intracellular dynamics have
                      been less well investigated due to lack of respective
                      probes. In the current study we characterized the FRET-based
                      K+ biosensor lc-LysM GEPII 1.0 for its capacity to measure
                      intracellular [K+] changes in primary cultured neurons and
                      in mouse cortical neurons in vivo. We found that lc-LysM
                      GEPII 1.0 can resolve neuronal [K+] decreases in vitro
                      during seizure-like and intense optogenetically evoked
                      activity. [K+] changes during single action potentials could
                      not be recorded. We confirmed these findings in vivo by
                      expressing lc-LysM GEPII 1.0 in mouse cortical neurons and
                      performing 2-photon fluorescence lifetime imaging. We
                      observed an increase in the fluorescence lifetime of lc-LysM
                      GEPII 1.0 during periinfarct depolarizations, which
                      indicates a decrease in intracellular neuronal [K+]. Our
                      findings suggest that lc-LysM GEPII 1.0 can be used to
                      measure large changes in [K+] in neurons in vitro and in
                      vivo but requires optimization to resolve smaller changes as
                      observed during single action potentials.},
      keywords     = {Animals / Potassium: metabolism / Neurons: metabolism /
                      Mice / Biosensing Techniques: methods / Action Potentials /
                      Cells, Cultured / Fluorescence Resonance Energy Transfer:
                      methods / Optogenetics: methods / Potassium (NLM Chemicals)},
      cin          = {LMF},
      ddc          = {600},
      cid          = {I:(DE-2719)1040180},
      pnm          = {899 - ohne Topic (POF4-899)},
      pid          = {G:(DE-HGF)POF4-899},
      experiment   = {EXP:(DE-2719)LMF-20190308},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:38877089},
      pmc          = {pmc:PMC11178854},
      doi          = {10.1038/s41598-024-62993-1},
      url          = {https://pub.dzne.de/record/270295},
}