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| Home > Publications Database > Probing intracellular potassium dynamics in neurons with the genetically encoded sensor lc-LysM GEPII 1.0 in vitro and in vivo. > print |
| Home > Publications Database > Probing intracellular potassium dynamics in neurons with the genetically encoded sensor lc-LysM GEPII 1.0 in vitro and in vivo. > print |
| 001 | 270295 | ||
| 005 | 20240809090117.0 | ||
| 024 | 7 | _ | |a 10.1038/s41598-024-62993-1 |2 doi |
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| 037 | _ | _ | |a DZNE-2024-00767 |
| 041 | _ | _ | |a English |
| 082 | _ | _ | |a 600 |
| 100 | 1 | _ | |a Groschup, Bernhard |b 0 |
| 245 | _ | _ | |a Probing intracellular potassium dynamics in neurons with the genetically encoded sensor lc-LysM GEPII 1.0 in vitro and in vivo. |
| 260 | _ | _ | |a [London] |c 2024 |b Macmillan Publishers Limited, part of Springer Nature |
| 336 | 7 | _ | |a article |2 DRIVER |
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| 520 | _ | _ | |a Neuronal activity is accompanied by a net outflow of potassium ions (K+) from the intra- to the extracellular space. While extracellular [K+] changes during neuronal activity are well characterized, intracellular dynamics have been less well investigated due to lack of respective probes. In the current study we characterized the FRET-based K+ biosensor lc-LysM GEPII 1.0 for its capacity to measure intracellular [K+] changes in primary cultured neurons and in mouse cortical neurons in vivo. We found that lc-LysM GEPII 1.0 can resolve neuronal [K+] decreases in vitro during seizure-like and intense optogenetically evoked activity. [K+] changes during single action potentials could not be recorded. We confirmed these findings in vivo by expressing lc-LysM GEPII 1.0 in mouse cortical neurons and performing 2-photon fluorescence lifetime imaging. We observed an increase in the fluorescence lifetime of lc-LysM GEPII 1.0 during periinfarct depolarizations, which indicates a decrease in intracellular neuronal [K+]. Our findings suggest that lc-LysM GEPII 1.0 can be used to measure large changes in [K+] in neurons in vitro and in vivo but requires optimization to resolve smaller changes as observed during single action potentials. |
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| 650 | _ | 7 | |a Potassium |0 RWP5GA015D |2 NLM Chemicals |
| 650 | _ | 2 | |a Animals |2 MeSH |
| 650 | _ | 2 | |a Potassium: metabolism |2 MeSH |
| 650 | _ | 2 | |a Neurons: metabolism |2 MeSH |
| 650 | _ | 2 | |a Mice |2 MeSH |
| 650 | _ | 2 | |a Biosensing Techniques: methods |2 MeSH |
| 650 | _ | 2 | |a Action Potentials |2 MeSH |
| 650 | _ | 2 | |a Cells, Cultured |2 MeSH |
| 650 | _ | 2 | |a Fluorescence Resonance Energy Transfer: methods |2 MeSH |
| 650 | _ | 2 | |a Optogenetics: methods |2 MeSH |
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| 700 | 1 | _ | |a Calandra, Gian-Marco |0 P:(DE-2719)9001033 |b 1 |u dzne |
| 700 | 1 | _ | |a Raitmayr, Constanze |b 2 |
| 700 | 1 | _ | |a Shrouder, Joshua |b 3 |
| 700 | 1 | _ | |a Llovera, Gemma |b 4 |
| 700 | 1 | _ | |a Zaki, Asal Ghaffari |b 5 |
| 700 | 1 | _ | |a Burgstaller, Sandra |b 6 |
| 700 | 1 | _ | |a Bischof, Helmut |b 7 |
| 700 | 1 | _ | |a Eroglu, Emrah |b 8 |
| 700 | 1 | _ | |a Liesz, Arthur |b 9 |
| 700 | 1 | _ | |a Malli, Roland |b 10 |
| 700 | 1 | _ | |a Filser, Severin |0 P:(DE-2719)2810523 |b 11 |e Last author |u dzne |
| 700 | 1 | _ | |a Plesnila, Nikolaus |b 12 |
| 773 | _ | _ | |a 10.1038/s41598-024-62993-1 |g Vol. 14, no. 1, p. 13753 |0 PERI:(DE-600)2615211-3 |n 1 |p 13753 |t Scientific reports |v 14 |y 2024 |x 2045-2322 |
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