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000270878 0247_ $$2doi$$a10.5281/ZENODO.7120626
000270878 037__ $$aDZNE-2024-00917
000270878 1001_ $$0P:(DE-2719)2810270$$aBradke, Frank$$b0$$eFirst author
000270878 245__ $$aSoftware: Microtubule retrograde flow retains neuronal polarization in a fluctuating state, v2
000270878 260__ $$aZenodo$$c2022
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000270878 520__ $$aIn developing vertebrate neurons, a neurite is formed by more than a hundred microtubules. While individual microtubules are dynamic, the microtubule array has been regarded as stationary. Using live-cell imaging of neurons in culture or in brain slices, combined with photoconversion techniques and pharmacological manipulations, we uncovered that the microtubule array flows retrogradely within neurites to the soma. This flow drives cycles of microtubule density, a hallmark of the fluctuating state before axon formation, thereby inhibiting neurite growth. The motor protein dynein fuels this process. Shortly after axon formation, microtubule retrograde flow slows down in the axon, reducing microtubule density cycles and enabling axon extension. Thus, keeping neurites short is an active process. Microtubule retrograde flow is a novel type of cytoskeletal dynamics, which changes the hitherto axon-centric view of neuronal polarization.
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000270878 650_7 $$2Other$$aNeurons
000270878 650_7 $$2Other$$acell biology
000270878 650_7 $$2Other$$acytoskeleton
000270878 650_7 $$2Other$$amicrotubules
000270878 650_7 $$2Other$$aLive cell imaging
000270878 650_7 $$2Other$$aPython
000270878 650_7 $$2Other$$aautomated analysis
000270878 650_7 $$2Other$$amicroscopy
000270878 650_7 $$2Other$$aDynein
000270878 650_7 $$2Other$$afigureflow
000270878 7001_ $$0P:(DE-2719)2811316$$aSchelski, Max$$b1$$eLast author$$udzne
000270878 773__ $$a10.5281/ZENODO.7120626
000270878 7870_ $$0DZNE-2023-00851$$aBradke, Frank et.al.$$dZenodo, 2022$$iRelatedTo$$r$$tSoftware: Microtubule retrograde flow retains neuronal polarization in a fluctuating state (v1)
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000270878 9141_ $$y2022
000270878 9201_ $$0I:(DE-2719)1013002$$kAG Bradke$$lAxon Growth and Regeneration$$x0
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