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@ARTICLE{Masala:270888,
      author       = {Masala, Nicola and Mittag, Manuel and Ambrad Giovannetti,
                      Eleonora and O'Neil, Darik A and Distler, Fabian J and
                      Rupprecht, Peter and Helmchen, Fritjof and Yuste, Rafael and
                      Fuhrmann, Martin and Beck, Heinz and Wenzel, Michael and
                      Kelly, Tony},
      title        = {{A}berrant hippocampal {C}a2+ microwaves following
                      synapsin-dependent adeno-associated viral expression of
                      {C}a2+ indicators.},
      journal      = {eLife},
      volume       = {13},
      issn         = {2050-084X},
      address      = {Cambridge},
      publisher    = {eLife Sciences Publications},
      reportid     = {DZNE-2024-00921},
      pages        = {RP93804},
      year         = {2024},
      abstract     = {Genetically encoded calcium indicators (GECIs) such as
                      GCaMP are invaluable tools in neuroscience to monitor
                      neuronal activity using optical imaging. The viral
                      transduction of GECIs is commonly used to target expression
                      to specific brain regions, can be conveniently used with any
                      mouse strain of interest without the need for prior crossing
                      with a GECI mouse line, and avoids potential hazards due to
                      the chronic expression of GECIs during development. A key
                      requirement for monitoring neuronal activity with an
                      indicator is that the indicator itself minimally affects
                      activity. Here, using common adeno-associated viral (AAV)
                      transduction procedures, we describe spatially confined
                      aberrant Ca2+ microwaves slowly travelling through the
                      hippocampus following expression of GCaMP6, GCaMP7, or
                      R-CaMP1.07 driven by the synapsin promoter with
                      AAV-dependent gene transfer in a titre-dependent fashion.
                      Ca2+ microwaves developed in hippocampal CA1 and CA3, but
                      not dentate gyrus nor neocortex, were typically first
                      observed at 4 wk after viral transduction, and persisted up
                      to at least 8 wk. The phenomenon was robust and observed
                      across laboratories with various experimenters and setups.
                      Our results indicate that aberrant hippocampal Ca2+
                      microwaves depend on the promoter and viral titre of the
                      GECI, density of expression, as well as the targeted brain
                      region. We used an alternative viral transduction method of
                      GCaMP which avoids this artefact. The results show that
                      commonly used Ca2+-indicator AAV transduction procedures can
                      produce artefactual Ca2+ responses. Our aim is to raise
                      awareness in the field of these artefactual
                      transduction-induced Ca2+ microwaves, and we provide a
                      potential solution.},
      keywords     = {Animals / Dependovirus: genetics / Synapsins: metabolism /
                      Synapsins: genetics / Calcium: metabolism / Hippocampus:
                      metabolism / Mice / Genetic Vectors / Transduction, Genetic
                      / Promoter Regions, Genetic / Mice, Inbred C57BL / Male /
                      AAV (Other) / GCaMP (Other) / GECI (Other) / in vivo (Other)
                      / mouse (Other) / neuroscience (Other) / Synapsins (NLM
                      Chemicals) / Calcium (NLM Chemicals)},
      cin          = {AG Fuhrmann},
      ddc          = {600},
      cid          = {I:(DE-2719)1011004},
      pnm          = {352 - Disease Mechanisms (POF4-352)},
      pid          = {G:(DE-HGF)POF4-352},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:39042440},
      pmc          = {pmc:PMC11265795},
      doi          = {10.7554/eLife.93804},
      url          = {https://pub.dzne.de/record/270888},
}