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@ARTICLE{Lehmann:271071,
author = {Lehmann, Johannes and Aly, Amr and Steffke, Christina and
Fabbio, Luca and Mayer, Valentin and Dikwella, Natalie and
Halablab, Kareen and Roselli, Francesco and Seiffert, Simone
and Boeckers, Tobias M and Brenner, David and Kabashi, Edor
and Mulaw, Medhanie and Ho, Ritchie and Catanese, Alberto},
title = {{H}eterozygous knockout of {S}ynaptotagmin13 phenocopies
{ALS} features and {TP}53 activation in human motor
neurons.},
journal = {Cell death $\&$ disease},
volume = {15},
number = {8},
issn = {2041-4889},
address = {London [u.a.]},
publisher = {Nature Publishing Group},
reportid = {DZNE-2024-00943},
pages = {560},
year = {2024},
abstract = {Spinal motor neurons (MNs) represent a highly vulnerable
cellular population, which is affected in fatal
neurodegenerative diseases such as amyotrophic lateral
sclerosis (ALS) and spinal muscular atrophy (SMA). In this
study, we show that the heterozygous loss of SYT13 is
sufficient to trigger a neurodegenerative phenotype
resembling those observed in ALS and SMA. SYT13+/-
hiPSC-derived MNs displayed a progressive manifestation of
typical neurodegenerative hallmarks such as loss of synaptic
contacts and accumulation of aberrant aggregates. Moreover,
analysis of the SYT13+/- transcriptome revealed a
significant impairment in biological mechanisms involved in
motoneuron specification and spinal cord differentiation.
This transcriptional portrait also strikingly correlated
with ALS signatures, displaying a significant convergence
toward the expression of pro-apoptotic and pro-inflammatory
genes, which are controlled by the transcription factor
TP53. Our data show for the first time that the heterozygous
loss of a single member of the synaptotagmin family, SYT13,
is sufficient to trigger a series of abnormal alterations
leading to MN sufferance, thus revealing novel insights into
the selective vulnerability of this cell population.},
keywords = {Humans / Tumor Suppressor Protein p53: metabolism / Tumor
Suppressor Protein p53: genetics / Motor Neurons: metabolism
/ Motor Neurons: pathology / Synaptotagmins: metabolism /
Synaptotagmins: genetics / Amyotrophic Lateral Sclerosis:
genetics / Amyotrophic Lateral Sclerosis: metabolism /
Amyotrophic Lateral Sclerosis: pathology / Heterozygote /
Phenotype / Induced Pluripotent Stem Cells: metabolism /
Induced Pluripotent Stem Cells: pathology / Cell
Differentiation: genetics / Gene Knockout Techniques / Tumor
Suppressor Protein p53 (NLM Chemicals) / Synaptotagmins (NLM
Chemicals) / TP53 protein, human (NLM Chemicals)},
cin = {AG Böckers / AG Roselli / Clinical Study Center Ulm ;
Clinical Study Center (Ulm)},
ddc = {570},
cid = {I:(DE-2719)1910002 / I:(DE-2719)1910001 /
I:(DE-2719)5000077},
pnm = {353 - Clinical and Health Care Research (POF4-353) / 352 -
Disease Mechanisms (POF4-352)},
pid = {G:(DE-HGF)POF4-353 / G:(DE-HGF)POF4-352},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:39097602},
pmc = {pmc:PMC11297993},
doi = {10.1038/s41419-024-06957-3},
url = {https://pub.dzne.de/record/271071},
}