TY  - JOUR
AU  - Dowling, Paul
AU  - Gargan, Stephen
AU  - Zweyer, Margit
AU  - Henry, Michael
AU  - Meleady, Paula
AU  - Swandulla, Dieter
AU  - Ohlendieck, Kay
TI  - Proteomic reference map for sarcopenia research: mass spectrometric identification of key muscle proteins located in the sarcomere, cytoskeleton and the extracellular matrix.
JO  - European journal of translational myology
VL  - 34
IS  - 2
SN  - 2037-7452
CY  - Padova
PB  - Unipress
M1  - DZNE-2024-01003
SP  - 12564
PY  - 2024
AB  - Sarcopenia of old age is characterized by the progressive loss of skeletal muscle mass and concomitant decrease in contractile strength. Age-related skeletal muscle dysfunctions play a key pathophysiological role in the frailty syndrome and can result in a drastically diminished quality of life in the elderly. Here we have used mass spectrometric analysis of the mouse hindlimb musculature to establish the muscle protein constellation at advanced age of a widely used sarcopenic animal model. Proteomic results were further analyzed by systems bioinformatics of voluntary muscles. In this report, the proteomic survey of aged muscles has focused on the expression patterns of proteins involved in the contraction-relaxation cycle, membrane cytoskeletal maintenance and the formation of the extracellular matrix. This includes proteomic markers of the fast versus slow phenotypes of myosin-containing thick filaments and actin-containing thin filaments, as well as proteins that are associated with the non-sarcomeric cytoskeleton and various matrisomal layers. The bioanalytical usefulness of the newly established reference map was demonstrated by the comparative screening of normal versus dystrophic muscles of old age, and findings were verified by immunoblot analysis.
LB  - PUB:(DE-HGF)16
C6  - pmid:38787300
C2  - pmc:PMC11264229
DO  - DOI:10.4081/ejtm.2024.12564
UR  - https://pub.dzne.de/record/271135
ER  -