000271283 001__ 271283
000271283 005__ 20240827163745.0
000271283 0247_ $$2doi$$a10.1515/mim-2024-0001
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000271283 037__ $$aDZNE-2024-01022
000271283 041__ $$aEnglish
000271283 1001_ $$aMicheva, Kristina D$$b0
000271283 245__ $$aArray tomography: trails to discovery.
000271283 260__ $$c2024
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000271283 500__ $$aMissing Journal: Methods in Microscopy () = 2942-3899 (import from CrossRef, PubMed, , Journals: pub.dzne.de)
000271283 520__ $$aTissue slicing is at the core of many approaches to studying biological structures. Among the modern volume electron microscopy (vEM) methods, array tomography (AT) is based on serial ultramicrotomy, section collection onto solid support, imaging via light and/or scanning electron microscopy, and re-assembly of the serial images into a volume for analysis. While AT largely uses standard EM equipment, it provides several advantages, including long-term preservation of the sample and compatibility with multi-scale and multi-modal imaging. Furthermore, the collection of serial ultrathin sections improves axial resolution and provides access for molecular labeling, which is beneficial for light microscopy and immunolabeling, and facilitates correlation with EM. Despite these benefits, AT techniques are underrepresented in imaging facilities and labs, due to their perceived difficulty and lack of training opportunities. Here we point towards novel developments in serial sectioning and image analysis that facilitate the AT pipeline, and solutions to overcome constraints. Because no single vEM technique can serve all needs regarding field of view and resolution, we sketch a decision tree to aid researchers in navigating the plethora of options available. Lastly, we elaborate on the unexplored potential of AT approaches to add valuable insight in diverse biological fields.
000271283 536__ $$0G:(DE-HGF)POF4-351$$a351 - Brain Function (POF4-351)$$cPOF4-351$$fPOF IV$$x0
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000271283 650_7 $$2Other$$aATUM
000271283 650_7 $$2Other$$aarray tomography
000271283 650_7 $$2Other$$alight microscopy
000271283 650_7 $$2Other$$aserial sectioning
000271283 650_7 $$2Other$$aultramicrotomy
000271283 650_7 $$2Other$$avolume electron microscopy
000271283 7001_ $$aBurden, Jemima J$$b1
000271283 7001_ $$0P:(DE-2719)2812260$$aSchifferer, Martina$$b2$$eLast author$$udzne
000271283 770__ $$aVolume Microscopy Across Scales
000271283 773__ $$a10.1515/mim-2024-0001$$gVol. 1, no. 1, p. 9 - 17$$n1$$p9 - 17$$v1$$y2024
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000271283 9101_ $$0I:(DE-588)1065079516$$6P:(DE-2719)2812260$$aDeutsches Zentrum für Neurodegenerative Erkrankungen$$b2$$kDZNE
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000271283 9141_ $$y2024
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000271283 915__ $$0LIC:(DE-HGF)CCBY4$$2HGFVOC$$aCreative Commons Attribution CC BY 4.0
000271283 9201_ $$0I:(DE-2719)1110000-4$$kAG Misgeld$$lNeuronal Cell Biology$$x0
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