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@ARTICLE{Micheva:271283,
      author       = {Micheva, Kristina D and Burden, Jemima J and Schifferer,
                      Martina},
      title        = {{A}rray tomography: trails to discovery.},
      volume       = {1},
      number       = {1},
      reportid     = {DZNE-2024-01022},
      pages        = {9 - 17},
      year         = {2024},
      note         = {Missing Journal: Methods in Microscopy () = 2942-3899
                      (import from CrossRef, PubMed, , Journals: pub.dzne.de)},
      abstract     = {Tissue slicing is at the core of many approaches to
                      studying biological structures. Among the modern volume
                      electron microscopy (vEM) methods, array tomography (AT) is
                      based on serial ultramicrotomy, section collection onto
                      solid support, imaging via light and/or scanning electron
                      microscopy, and re-assembly of the serial images into a
                      volume for analysis. While AT largely uses standard EM
                      equipment, it provides several advantages, including
                      long-term preservation of the sample and compatibility with
                      multi-scale and multi-modal imaging. Furthermore, the
                      collection of serial ultrathin sections improves axial
                      resolution and provides access for molecular labeling, which
                      is beneficial for light microscopy and immunolabeling, and
                      facilitates correlation with EM. Despite these benefits, AT
                      techniques are underrepresented in imaging facilities and
                      labs, due to their perceived difficulty and lack of training
                      opportunities. Here we point towards novel developments in
                      serial sectioning and image analysis that facilitate the AT
                      pipeline, and solutions to overcome constraints. Because no
                      single vEM technique can serve all needs regarding field of
                      view and resolution, we sketch a decision tree to aid
                      researchers in navigating the plethora of options available.
                      Lastly, we elaborate on the unexplored potential of AT
                      approaches to add valuable insight in diverse biological
                      fields.},
      keywords     = {ATUM (Other) / array tomography (Other) / light microscopy
                      (Other) / serial sectioning (Other) / ultramicrotomy (Other)
                      / volume electron microscopy (Other)},
      cin          = {AG Misgeld},
      cid          = {I:(DE-2719)1110000-4},
      pnm          = {351 - Brain Function (POF4-351)},
      pid          = {G:(DE-HGF)POF4-351},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:39119254},
      pmc          = {pmc:PMC11308915},
      doi          = {10.1515/mim-2024-0001},
      url          = {https://pub.dzne.de/record/271283},
}