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001     271283
005     20240827163745.0
024 7 _ |a 10.1515/mim-2024-0001
|2 doi
024 7 _ |a pmid:39119254
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024 7 _ |a pmc:PMC11308915
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037 _ _ |a DZNE-2024-01022
041 _ _ |a English
100 1 _ |a Micheva, Kristina D
|b 0
245 _ _ |a Array tomography: trails to discovery.
260 _ _ |c 2024
336 7 _ |a article
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336 7 _ |a Output Types/Journal article
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336 7 _ |a Journal Article
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336 7 _ |a ARTICLE
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336 7 _ |a JOURNAL_ARTICLE
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336 7 _ |a Journal Article
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500 _ _ |a Missing Journal: Methods in Microscopy () = 2942-3899 (import from CrossRef, PubMed, , Journals: pub.dzne.de)
520 _ _ |a Tissue slicing is at the core of many approaches to studying biological structures. Among the modern volume electron microscopy (vEM) methods, array tomography (AT) is based on serial ultramicrotomy, section collection onto solid support, imaging via light and/or scanning electron microscopy, and re-assembly of the serial images into a volume for analysis. While AT largely uses standard EM equipment, it provides several advantages, including long-term preservation of the sample and compatibility with multi-scale and multi-modal imaging. Furthermore, the collection of serial ultrathin sections improves axial resolution and provides access for molecular labeling, which is beneficial for light microscopy and immunolabeling, and facilitates correlation with EM. Despite these benefits, AT techniques are underrepresented in imaging facilities and labs, due to their perceived difficulty and lack of training opportunities. Here we point towards novel developments in serial sectioning and image analysis that facilitate the AT pipeline, and solutions to overcome constraints. Because no single vEM technique can serve all needs regarding field of view and resolution, we sketch a decision tree to aid researchers in navigating the plethora of options available. Lastly, we elaborate on the unexplored potential of AT approaches to add valuable insight in diverse biological fields.
536 _ _ |a 351 - Brain Function (POF4-351)
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588 _ _ |a Dataset connected to CrossRef, PubMed, , Journals: pub.dzne.de
650 _ 7 |a ATUM
|2 Other
650 _ 7 |a array tomography
|2 Other
650 _ 7 |a light microscopy
|2 Other
650 _ 7 |a serial sectioning
|2 Other
650 _ 7 |a ultramicrotomy
|2 Other
650 _ 7 |a volume electron microscopy
|2 Other
700 1 _ |a Burden, Jemima J
|b 1
700 1 _ |a Schifferer, Martina
|0 P:(DE-2719)2812260
|b 2
|e Last author
|u dzne
770 _ _ |a Volume Microscopy Across Scales
773 _ _ |a 10.1515/mim-2024-0001
|g Vol. 1, no. 1, p. 9 - 17
|n 1
|p 9 - 17
|v 1
|y 2024
856 4 _ |y OpenAccess
|u https://pub.dzne.de/record/271283/files/DZNE-2024-01022.pdf
856 4 _ |y OpenAccess
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910 1 _ |a Deutsches Zentrum für Neurodegenerative Erkrankungen
|0 I:(DE-588)1065079516
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913 1 _ |a DE-HGF
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914 1 _ |y 2024
915 _ _ |a OpenAccess
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915 _ _ |a Creative Commons Attribution CC BY 4.0
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920 1 _ |0 I:(DE-2719)1110000-4
|k AG Misgeld
|l Neuronal Cell Biology
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980 _ _ |a journal
980 _ _ |a VDB
980 _ _ |a UNRESTRICTED
980 _ _ |a I:(DE-2719)1110000-4
980 1 _ |a FullTexts

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