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@ARTICLE{Cangalaya:271336,
      author       = {Cangalaya, Carla and Sun, Weilun and Stoyanov, Stoyan
                      Borislavov and Dunay, Ildiko Rita and Dityatev, Alexander},
      title        = {{I}ntegrity of neural extracellular matrix is required for
                      microglia-mediated synaptic remodeling.},
      journal      = {Glia},
      volume       = {72},
      number       = {10},
      issn         = {0894-1491},
      address      = {Bognor Regis [u.a.]},
      publisher    = {Wiley-Liss},
      reportid     = {DZNE-2024-01038},
      pages        = {1874 - 1892},
      year         = {2024},
      abstract     = {Microglia continuously remodel synapses, which are embedded
                      in the extracellular matrix (ECM). However, the mechanisms,
                      which govern this process remain elusive. To investigate the
                      influence of the neural ECM in synaptic remodeling by
                      microglia, we disrupted ECM integrity by injection of
                      chondroitinase ABC (ChABC) into the retrosplenial cortex of
                      healthy adult mice. Using in vivo two-photon microscopy we
                      found that ChABC treatment increased microglial branching
                      complexity and ECM phagocytic capacity and decreased spine
                      elimination rate under basal conditions. Moreover, ECM
                      attenuation largely prevented synaptic remodeling following
                      synaptic stress induced by photodamage of single synaptic
                      elements. These changes were associated with less stable and
                      smaller microglial contacts at the synaptic damage sites,
                      diminished deposition of calreticulin and complement
                      proteins C1q and C3 at synapses and impaired expression of
                      microglial CR3 receptor. Thus, our findings provide novel
                      insights into the function of the neural ECM in deposition
                      of complement proteins and synaptic remodeling by
                      microglia.},
      keywords     = {Animals / Microglia: metabolism / Microglia: drug effects /
                      Extracellular Matrix: metabolism / Extracellular Matrix:
                      drug effects / Synapses: metabolism / Synapses: drug effects
                      / Synapses: physiology / Complement C1q: metabolism / Mice,
                      Inbred C57BL / Chondroitin ABC Lyase: pharmacology / Mice /
                      Neuronal Plasticity: physiology / Neuronal Plasticity: drug
                      effects / Complement C3: metabolism / Calreticulin:
                      metabolism / Male / Phagocytosis: physiology / Phagocytosis:
                      drug effects / Mice, Transgenic / Macrophage-1 Antigen:
                      metabolism / C1q (Other) / C3 (Other) / complement protein
                      (Other) / extracellular matrix (Other) / microglia (Other) /
                      spine (Other) / synapse (Other) / two‐photon microscopy
                      (Other) / Complement C1q (NLM Chemicals) / Chondroitin ABC
                      Lyase (NLM Chemicals) / Complement C3 (NLM Chemicals) /
                      Calreticulin (NLM Chemicals) / Macrophage-1 Antigen (NLM
                      Chemicals)},
      cin          = {AG Dityatev},
      ddc          = {610},
      cid          = {I:(DE-2719)1310007},
      pnm          = {351 - Brain Function (POF4-351)},
      pid          = {G:(DE-HGF)POF4-351},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:38946065},
      doi          = {10.1002/glia.24588},
      url          = {https://pub.dzne.de/record/271336},
}