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@INBOOK{Crockett:271880,
author = {Crockett, Alexis and Fuhrmann, Martin and Garaschuk, Olga
and Davalos, Dimitrios},
title = {{P}rogress in {S}tructural and {F}unctional {I}n {V}ivo
{I}maging of {M}icroglia and {T}heir {A}pplication in
{H}ealth and {D}isease.},
volume = {37},
address = {Cham},
publisher = {Springer International Publishing},
reportid = {DZNE-2024-01092},
isbn = {978-3-031-55528-2 (print)},
series = {Advances in Neurobiology},
pages = {65 - 80},
year = {2024},
comment = {Microglia / Tremblay, Marie-Ève (Editor) ; Cham : Springer
International Publishing, 2024, Chapter 5 ; ISSN:
2190-5215=2190-5223 ; ISBN:
978-3-031-55528-2=978-3-031-55529-9 ;
doi:10.1007/978-3-031-55529-9},
booktitle = {Microglia / Tremblay, Marie-Ève
(Editor) ; Cham : Springer
International Publishing, 2024, Chapter
5 ; ISSN: 2190-5215=2190-5223 ; ISBN:
978-3-031-55528-2=978-3-031-55529-9 ;
doi:10.1007/978-3-031-55529-9},
abstract = {The first line of defense for the central nervous system
(CNS) against injury or disease is provided by microglia.
Microglia were long believed to stay in a dormant/resting
state, reacting only to injury or disease. This view changed
dramatically with the development of modern imaging
techniques that allowed the study of microglial behavior in
the intact brain over time, to reveal the dynamic nature of
their responses. Over the past two decades, in vivo imaging
using multiphoton microscopy has revealed numerous new
functions of microglia in the developing, adult, aged,
injured, and diseased CNS. As the most dynamic cells in the
brain, microglia continuously contact all structures and
cell types, such as glial and vascular cells, neuronal cell
bodies, axons, dendrites, and dendritic spines, and are
believed to play a central role in sculpting neuronal
networks throughout life. Following trauma, or in
neurodegenerative or neuroinflammatory diseases, microglial
responses range from protective to harmful, underscoring the
need to better understand their diverse roles and states in
different pathological conditions. In this chapter, we
introduce multiphoton microscopy and discuss recent advances
in structural and functional imaging technologies that have
expanded our toolbox to study microglial states and
behaviors in new ways and depths. We also discuss relevant
mouse models available for in vivo imaging studies of
microglia and review how such studies are constantly
refining our understanding of the multifaceted role of
microglia in the healthy and diseased CNS.},
keywords = {Microglia: metabolism / Microglia: pathology / Animals /
Humans / Microscopy, Fluorescence, Multiphoton / Brain:
diagnostic imaging / Neuroinflammatory Diseases: diagnostic
imaging / Neuroinflammatory Diseases: pathology /
Neurodegenerative Diseases: diagnostic imaging /
Neurodegenerative Diseases: pathology / Alzheimer’s
disease (Other) / Imaging technologies and methods (Other) /
In vitro experiments (Other) / In vivo imaging (Other) /
Microglia (Other) / Mouse models (Other) / Multiple
sclerosis (Other) / Two-photon microscopy (Other)},
cin = {AG Fuhrmann},
cid = {I:(DE-2719)1011004},
pnm = {352 - Disease Mechanisms (POF4-352)},
pid = {G:(DE-HGF)POF4-352},
typ = {PUB:(DE-HGF)7},
pubmed = {pmid:39207687},
doi = {10.1007/978-3-031-55529-9_5},
url = {https://pub.dzne.de/record/271880},
}