TY  - CHAP
AU  - Zeug, André
AU  - Unichenko, Petr
AU  - Müller, Franziska E.
AU  - Henneberger, Christian
A3  - Rusakov, Dmitri
TI  - Quantitative Optical Analysis of Astrocytic Ca2+ Signaling
VL  - 209
CY  - New York, NY
PB  - Springer US
M1  - DZNE-2024-01157
SN  - 978-1-0716-4010-4 (print)
T2  - Neuromethods
SP  - 21 - 57
PY  - 2024
AB  - Ca2+ signaling of astrocytes plays a central role in the physiology and pathophysiology of these non-neuronal cells in the brain. Decades of research have revealed the heterogeneity of astrocytic Ca2+ signals and how they are triggered, how they propagate, what mechanisms sustain them, and what their downstream effects are on the cellular, network, and behavioral level. Imaging of astrocytic Ca2+ signals has been and is at the center of this research. It has benefited from the development of novel indicators, of new imaging techniques, and of new analytical approaches. This enabled researchers to uncover a striking diversity of Ca2+ signals regarding, for instance, their spatial and temporal patterns, which have been linked to the operation of brain circuits. When interpreting recorded Ca2+ signals it needs to be considered how cellular changes of [Ca2+] are reflected by the recorded fluorescence of a Ca2+ indicator, what available and useful analytical approaches are, and what their advantages and limitations are. Here, we provide an overview of quantitative Ca2+ imaging in astrocytes by covering theoretical and practical aspects of Ca2+ imaging, available indicators and strategies, and toolboxes for image data analysis.
LB  - PUB:(DE-HGF)7
DO  - DOI:10.1007/978-1-0716-4011-1_2
UR  - https://pub.dzne.de/record/272338
ER  -