TY  - JOUR
AU  - Bartos, Laura M.
AU  - Kunte, Sebastian T.
AU  - Wagner, Stephan
AU  - Beumers, Philipp
AU  - Schaefer, Rebecca
AU  - Zatcepin, Artem
AU  - Li, Yunlei
AU  - Griessl, Maria
AU  - Hoermann, Leonie
AU  - Wind-Mark, Karin
AU  - Bartenstein, Peter
AU  - Tahirovic, Sabina
AU  - Ziegler, Sibylle
AU  - Brendel, Matthias
AU  - Gnörich, Johannes
TI  - Astroglial glucose uptake determines brain FDG-PET alterations and metabolic connectivity during healthy aging in mice
JO  - NeuroImage
VL  - 300
SN  - 1053-8119
CY  - Orlando, Fla.
PB  - Academic Press
M1  - DZNE-2024-01186
SP  - 120860
PY  - 2024
AB  -  2-Fluorodeoxyglucose-PET (FDG-PET) is a powerful tool to study glucose metabolism in mammalian brains, but cellular sources of glucose uptake and metabolic connectivity during aging are not yet understood.Healthy wild-type mice of both sexes (2-21 months of age) received FDG-PET and cell sorting after in vivo tracer injection (scRadiotracing). FDG uptake per cell was quantified in isolated microglia, astrocytes and neurons. Cerebral FDG uptake and metabolic connectivity were determined by PET. A subset of mice received measurement of blood glucose levels to study associations with cellular FDG uptake during aging.Cerebral FDG-PET signals in healthy mice increased linearly with age. Cellular FDG uptake of neurons increased between 2 and 12 months of age, followed by a strong decrease towards late ages. Contrarily, FDG uptake in microglia and astrocytes exhibited a U-shaped function with respect to age, comprising the predominant cellular source of higher cerebral FDG uptake in the later stages. Metabolic connectivity was closely associated with the ratio of glucose uptake in astroglial cells relative to neurons. Cellular FDG uptake was not associated with blood glucose levels and increasing FDG brain uptake as a function of age was still observed after adjusting for blood glucose levels.Trajectories of astroglial glucose uptake drive brain FDG-PET alterations and metabolic connectivity during aging.
KW  - Animals
KW  - Fluorodeoxyglucose F18: pharmacokinetics
KW  - Astrocytes: metabolism
KW  - Positron-Emission Tomography: methods
KW  - Mice
KW  - Glucose: metabolism
KW  - Male
KW  - Brain: metabolism
KW  - Brain: diagnostic imaging
KW  - Female
KW  - Mice, Inbred C57BL
KW  - Aging: metabolism
KW  - Radiopharmaceuticals: pharmacokinetics
KW  - Neurons: metabolism
KW  - Healthy Aging: metabolism
KW  - Microglia: metabolism
KW  - Aging (Other)
KW  - Astroglia (Other)
KW  - FDG-PET (Other)
KW  - Metabolic connectivity (Other)
KW  - Scradiotracing (Other)
KW  - Fluorodeoxyglucose F18 (NLM Chemicals)
KW  - Glucose (NLM Chemicals)
KW  - Radiopharmaceuticals (NLM Chemicals)
LB  - PUB:(DE-HGF)16
C6  - pmid:39332748
DO  - DOI:10.1016/j.neuroimage.2024.120860
UR  - https://pub.dzne.de/record/272510
ER  -