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@ARTICLE{Bartos:272510,
author = {Bartos, Laura M. and Kunte, Sebastian T. and Wagner,
Stephan and Beumers, Philipp and Schaefer, Rebecca and
Zatcepin, Artem and Li, Yunlei and Griessl, Maria and
Hoermann, Leonie and Wind-Mark, Karin and Bartenstein, Peter
and Tahirovic, Sabina and Ziegler, Sibylle and Brendel,
Matthias and Gnörich, Johannes},
title = {{A}stroglial glucose uptake determines brain {FDG}-{PET}
alterations and metabolic connectivity during healthy aging
in mice},
journal = {NeuroImage},
volume = {300},
issn = {1053-8119},
address = {Orlando, Fla.},
publisher = {Academic Press},
reportid = {DZNE-2024-01186},
pages = {120860},
year = {2024},
abstract = {2-Fluorodeoxyglucose-PET (FDG-PET) is a powerful tool to
study glucose metabolism in mammalian brains, but cellular
sources of glucose uptake and metabolic connectivity during
aging are not yet understood.Healthy wild-type mice of both
sexes (2-21 months of age) received FDG-PET and cell sorting
after in vivo tracer injection (scRadiotracing). FDG uptake
per cell was quantified in isolated microglia, astrocytes
and neurons. Cerebral FDG uptake and metabolic connectivity
were determined by PET. A subset of mice received
measurement of blood glucose levels to study associations
with cellular FDG uptake during aging.Cerebral FDG-PET
signals in healthy mice increased linearly with age.
Cellular FDG uptake of neurons increased between 2 and 12
months of age, followed by a strong decrease towards late
ages. Contrarily, FDG uptake in microglia and astrocytes
exhibited a U-shaped function with respect to age,
comprising the predominant cellular source of higher
cerebral FDG uptake in the later stages. Metabolic
connectivity was closely associated with the ratio of
glucose uptake in astroglial cells relative to neurons.
Cellular FDG uptake was not associated with blood glucose
levels and increasing FDG brain uptake as a function of age
was still observed after adjusting for blood glucose
levels.Trajectories of astroglial glucose uptake drive brain
FDG-PET alterations and metabolic connectivity during
aging.},
keywords = {Animals / Fluorodeoxyglucose F18: pharmacokinetics /
Astrocytes: metabolism / Positron-Emission Tomography:
methods / Mice / Glucose: metabolism / Male / Brain:
metabolism / Brain: diagnostic imaging / Female / Mice,
Inbred C57BL / Aging: metabolism / Radiopharmaceuticals:
pharmacokinetics / Neurons: metabolism / Healthy Aging:
metabolism / Microglia: metabolism / Aging (Other) /
Astroglia (Other) / FDG-PET (Other) / Metabolic connectivity
(Other) / Scradiotracing (Other) / Fluorodeoxyglucose F18
(NLM Chemicals) / Glucose (NLM Chemicals) /
Radiopharmaceuticals (NLM Chemicals)},
cin = {AG Haass / AG Tahirovic},
ddc = {610},
cid = {I:(DE-2719)1110007 / I:(DE-2719)1140003},
pnm = {352 - Disease Mechanisms (POF4-352)},
pid = {G:(DE-HGF)POF4-352},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:39332748},
doi = {10.1016/j.neuroimage.2024.120860},
url = {https://pub.dzne.de/record/272510},
}