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000272574 005__ 20250120102326.0
000272574 0247_ $$2doi$$a10.7554/eLife.87078.1
000272574 037__ $$aDZNE-2024-01195
000272574 1001_ $$00000-0002-3344-8683$$aThome, Christian$$b0
000272574 245__ $$aLive imaging of excitable axonal microdomains in ankyrin-G-GFP mice
000272574 260__ $$c2023
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000272574 520__ $$aThe axon initial segment (AIS) constitutes not only the site of action potential initiation, but also a hub for activity-dependent modulation of output generation. Recent studies shedding light on AIS function used predominantly post hoc approaches since no robust murine in vivo live reporters exist. Here, we introduce a reporter line in which the AIS is intrinsically labeled by an ankyrin-G-GFP fusion protein activated by Cre recombinase, tagging the native Ank3 gene. Using confocal, superresolution, and two-photon microscopy as well as whole-cell patch-clamp recordings in vitro, ex vivo, and in vivo, we confirm that the subcellular scaffold of the AIS and electrophysiological parameters of labelled cells remain unchanged. We further uncover rapid AIS remodeling following increased network activity in this model system, as well as highly reproducible in vivo labeling of AIS over weeks. This novel reporter line allows longitudinal studies of AIS modulation and plasticity in vivo in real time and thus provides a unique approach to study subcellular plasticity in a broad range of applications.
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000272574 7001_ $$00000-0002-3818-7910$$aJanssen, Jan Maximilian$$b1
000272574 7001_ $$aKarabulut, Seda$$b2
000272574 7001_ $$00000-0002-8849-6260$$aAcuna, Claudio$$b3
000272574 7001_ $$00000-0003-0781-187X$$aD’Este, Elisa$$b4
000272574 7001_ $$00000-0002-8070-8951$$aSoyka, Stella J.$$b5
000272574 7001_ $$aBaum, Konrad$$b6
000272574 7001_ $$aBock, Michael$$b7
000272574 7001_ $$00000-0003-4801-3057$$aLehmann, Nadja$$b8
000272574 7001_ $$0P:(DE-2719)9001582$$aHasegawa, Masashi$$b9$$udzne
000272574 7001_ $$0P:(DE-2719)9001367$$aGanea, Dan Alin$$b10$$udzne
000272574 7001_ $$0P:(DE-2719)9001347$$aBenoit, Chloe$$b11$$udzne
000272574 7001_ $$0P:(DE-2719)9001219$$aGründemann, Jan$$b12
000272574 7001_ $$00000-0002-9040-9891$$aSchultz, Christian$$b13
000272574 7001_ $$00000-0003-2695-7209$$aBennett, Vann$$b14
000272574 7001_ $$00000-0002-4207-5823$$aJenkins, Paul M.$$b15
000272574 7001_ $$00000-0001-8020-6604$$aEngelhardt, Maren$$b16
000272574 773__ $$a10.7554/eLife.87078.1
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000272574 9141_ $$y2024
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000272574 9201_ $$0I:(DE-2719)5000069$$kAG Gründemann$$lNeural Circuit Computations$$x0
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