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| 001 | 272574 | ||
| 005 | 20250120102326.0 | ||
| 024 | 7 | _ | |a 10.7554/eLife.87078.1 |2 doi |
| 037 | _ | _ | |a DZNE-2024-01195 |
| 100 | 1 | _ | |a Thome, Christian |0 0000-0002-3344-8683 |b 0 |
| 245 | _ | _ | |a Live imaging of excitable axonal microdomains in ankyrin-G-GFP mice |
| 260 | _ | _ | |c 2023 |
| 336 | 7 | _ | |a Preprint |b preprint |m preprint |0 PUB:(DE-HGF)25 |s 1728571429_13607 |2 PUB:(DE-HGF) |
| 336 | 7 | _ | |a WORKING_PAPER |2 ORCID |
| 336 | 7 | _ | |a Electronic Article |0 28 |2 EndNote |
| 336 | 7 | _ | |a preprint |2 DRIVER |
| 336 | 7 | _ | |a ARTICLE |2 BibTeX |
| 336 | 7 | _ | |a Output Types/Working Paper |2 DataCite |
| 520 | _ | _ | |a The axon initial segment (AIS) constitutes not only the site of action potential initiation, but also a hub for activity-dependent modulation of output generation. Recent studies shedding light on AIS function used predominantly post hoc approaches since no robust murine in vivo live reporters exist. Here, we introduce a reporter line in which the AIS is intrinsically labeled by an ankyrin-G-GFP fusion protein activated by Cre recombinase, tagging the native Ank3 gene. Using confocal, superresolution, and two-photon microscopy as well as whole-cell patch-clamp recordings in vitro, ex vivo, and in vivo, we confirm that the subcellular scaffold of the AIS and electrophysiological parameters of labelled cells remain unchanged. We further uncover rapid AIS remodeling following increased network activity in this model system, as well as highly reproducible in vivo labeling of AIS over weeks. This novel reporter line allows longitudinal studies of AIS modulation and plasticity in vivo in real time and thus provides a unique approach to study subcellular plasticity in a broad range of applications. |
| 536 | _ | _ | |a 351 - Brain Function (POF4-351) |0 G:(DE-HGF)POF4-351 |c POF4-351 |f POF IV |x 0 |
| 588 | _ | _ | |a Dataset connected to CrossRef |
| 700 | 1 | _ | |a Janssen, Jan Maximilian |0 0000-0002-3818-7910 |b 1 |
| 700 | 1 | _ | |a Karabulut, Seda |b 2 |
| 700 | 1 | _ | |a Acuna, Claudio |0 0000-0002-8849-6260 |b 3 |
| 700 | 1 | _ | |a D’Este, Elisa |0 0000-0003-0781-187X |b 4 |
| 700 | 1 | _ | |a Soyka, Stella J. |0 0000-0002-8070-8951 |b 5 |
| 700 | 1 | _ | |a Baum, Konrad |b 6 |
| 700 | 1 | _ | |a Bock, Michael |b 7 |
| 700 | 1 | _ | |a Lehmann, Nadja |0 0000-0003-4801-3057 |b 8 |
| 700 | 1 | _ | |a Hasegawa, Masashi |0 P:(DE-2719)9001582 |b 9 |u dzne |
| 700 | 1 | _ | |a Ganea, Dan Alin |0 P:(DE-2719)9001367 |b 10 |u dzne |
| 700 | 1 | _ | |a Benoit, Chloe |0 P:(DE-2719)9001347 |b 11 |u dzne |
| 700 | 1 | _ | |a Gründemann, Jan |0 P:(DE-2719)9001219 |b 12 |
| 700 | 1 | _ | |a Schultz, Christian |0 0000-0002-9040-9891 |b 13 |
| 700 | 1 | _ | |a Bennett, Vann |0 0000-0003-2695-7209 |b 14 |
| 700 | 1 | _ | |a Jenkins, Paul M. |0 0000-0002-4207-5823 |b 15 |
| 700 | 1 | _ | |a Engelhardt, Maren |0 0000-0001-8020-6604 |b 16 |
| 773 | _ | _ | |a 10.7554/eLife.87078.1 |
| 856 | 4 | _ | |y OpenAccess |u https://pub.dzne.de/record/272574/files/DZNE-2024-01195_Preprint.pdf |
| 856 | 4 | _ | |y OpenAccess |x pdfa |u https://pub.dzne.de/record/272574/files/DZNE-2024-01195_Preprint.pdf?subformat=pdfa |
| 909 | C | O | |o oai:pub.dzne.de:272574 |p openaire |p open_access |p VDB |p driver |p dnbdelivery |
| 910 | 1 | _ | |a Deutsches Zentrum für Neurodegenerative Erkrankungen |0 I:(DE-588)1065079516 |k DZNE |b 9 |6 P:(DE-2719)9001582 |
| 910 | 1 | _ | |a External Institute |0 I:(DE-HGF)0 |k Extern |b 10 |6 P:(DE-2719)9001367 |
| 910 | 1 | _ | |a Deutsches Zentrum für Neurodegenerative Erkrankungen |0 I:(DE-588)1065079516 |k DZNE |b 11 |6 P:(DE-2719)9001347 |
| 910 | 1 | _ | |a Deutsches Zentrum für Neurodegenerative Erkrankungen |0 I:(DE-588)1065079516 |k DZNE |b 12 |6 P:(DE-2719)9001219 |
| 913 | 1 | _ | |a DE-HGF |b Gesundheit |l Neurodegenerative Diseases |1 G:(DE-HGF)POF4-350 |0 G:(DE-HGF)POF4-351 |3 G:(DE-HGF)POF4 |2 G:(DE-HGF)POF4-300 |4 G:(DE-HGF)POF |v Brain Function |x 0 |
| 914 | 1 | _ | |y 2024 |
| 915 | _ | _ | |a OpenAccess |0 StatID:(DE-HGF)0510 |2 StatID |
| 915 | _ | _ | |a Creative Commons Attribution CC BY 4.0 |0 LIC:(DE-HGF)CCBY4 |2 HGFVOC |
| 920 | 1 | _ | |0 I:(DE-2719)5000069 |k AG Gründemann |l Neural Circuit Computations |x 0 |
| 980 | 1 | _ | |a FullTexts |
| 980 | _ | _ | |a preprint |
| 980 | _ | _ | |a VDB |
| 980 | _ | _ | |a UNRESTRICTED |
| 980 | _ | _ | |a I:(DE-2719)5000069 |
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