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@ARTICLE{LuczakSobotkowska:272577,
      author       = {Luczak-Sobotkowska, Zuzanna M and Rosa, Patrycja and Lopez,
                      Maria Banqueri and Ochocka, Natalia and Kiryk, Anna and
                      Lenkiewicz, Anna M and Fuhrmann, Martin and Jankowski,
                      Aleksander and Kaminska, Bozena},
      title        = {{T}racking changes in functionality and morphology of
                      repopulated microglia in young and old mice.},
      journal      = {Journal of neuroinflammation},
      volume       = {21},
      number       = {1},
      issn         = {1742-2094},
      address      = {London},
      publisher    = {BioMed Central},
      reportid     = {DZNE-2024-01198},
      pages        = {248},
      year         = {2024},
      abstract     = {Microglia (MG) are myeloid cells of the central nervous
                      system that support homeostasis and instigate
                      neuroinflammation in pathologies. Single-cell RNA sequencing
                      (scRNA-seq) revealed the functional heterogeneity of MG in
                      mouse brains. Microglia are self-renewing cells and
                      inhibition of colony-stimulating factor 1 receptor (CSF1R)
                      signaling depletes microglia which rapidly repopulate. The
                      functions of repopulated microglia are poorly known.We
                      combined scRNA-seq, bulk RNA-seq, immunofluorescence, and
                      confocal imaging to study the functionalities and morphology
                      of repopulated microglia.A CSRF1R inhibitor (BLZ-945)
                      depleted microglia within 21 days and a number of microglia
                      was fully restored within 7 days, as confirmed by TMEM119
                      staining and flow cytometry. ScRNA-seq and computational
                      analyses demonstrate that repopulated microglia originated
                      from preexisting progenitors and reconstituted functional
                      clusters but upregulated inflammatory genes. Percentages of
                      proliferating, immature microglia displaying inflammatory
                      gene expression increased in aging mice. Morphometric
                      analysis of MG cell body and branching revealed a distinct
                      morphology of repopulated MG, particularly in brains of old
                      mice. We demonstrate that with aging some repopulated MG
                      fail to reach the homeostatic phenotype. These differences
                      may contribute to the deterioration of MG protective
                      functions with age.},
      keywords     = {Animals / Microglia: metabolism / Mice / Aging: physiology
                      / Mice, Inbred C57BL / Brain: cytology / Brain: metabolism /
                      Male / Receptors, Granulocyte-Macrophage Colony-Stimulating
                      Factor: metabolism / Receptors, Granulocyte-Macrophage
                      Colony-Stimulating Factor: genetics / Single-Cell Analysis /
                      Aging (Other) / CSF1R inhibitors (Other) / Microglia
                      repopulation (Other) / Microglial heterogeneity (Other) /
                      ScRNA-seq (Other) / Transcriptomics (Other) / Receptors,
                      Granulocyte-Macrophage Colony-Stimulating Factor (NLM
                      Chemicals)},
      cin          = {AG Fuhrmann},
      ddc          = {610},
      cid          = {I:(DE-2719)1011004},
      pnm          = {352 - Disease Mechanisms (POF4-352)},
      pid          = {G:(DE-HGF)POF4-352},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:39363245},
      pmc          = {pmc:PMC11448401},
      doi          = {10.1186/s12974-024-03242-0},
      url          = {https://pub.dzne.de/record/272577},
}