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@ARTICLE{Blmeke:273930,
author = {Blömeke, Lara and Rehn, Fabian and Pils, Marlene and
Kraemer-Schulien, Victoria and Cousin, Anneliese and
Kutzsche, Janine and Bujnicki, Tuyen and Freiesleben, Silka
D and Schneider, Luisa-Sophie and Preis, Lukas and Priller,
Josef and Spruth, Eike Jakob and Altenstein, Slawek and
Schneider, Anja and Fliessbach, Klaus and Wiltfang, Jens and
Hansen, Niels and Rostamzadeh, Ayda and Düzel, Emrah and
Glanz, Wenzel and Incesoy, Enise I and Buerger, Katharina
and Janowitz, Daniel and Ewers, Michael and Perneczky,
Robert and Rauchmann, Boris Stephan and Teipel, Stefan and
Kilimann, Ingo and Laske, Christoph and Munk, Matthias H and
Spottke, Annika and Roy, Nina and Heneka, Michael and
Brosseron, Frederic and Wagner, Michael and Roeske, Sandra
and Ramirez, Alfredo and Schmid, Matthias and Jessen, Frank
and Bannach, Oliver and Peters, Oliver and Willbold, Dieter},
title = {{B}lood-based quantification of {A}β oligomers indicates
impaired clearance from brain in {A}po{E} ε4 positive
subjects.},
journal = {Communications medicine},
volume = {4},
number = {1},
issn = {2730-664X},
address = {[London]},
publisher = {Springer Nature},
reportid = {DZNE-2024-01404},
pages = {262},
year = {2024},
abstract = {Quantification of Amyloid beta (Aβ) oligomers in plasma
enables early diagnosis of Alzheimer's Disease (AD) and
improves our understanding of underlying pathologies.
However, quantification necessitates an extremely sensitive
and selective technology because of very low Aβ oligomer
concentrations and possible interference from matrix
components.In this report, we developed and validated a
surface-based fluorescence distribution analysis (sFIDA)
assay for quantification of Aβ oligomers in plasma.The
blood-based sFIDA assay delivers a sensitivity of 1.8 fM, an
inter- and intra-assay variation below $20\%$ for oligomer
calibration standards and no interference with matrix
components. Quantification of Aβ oligomers in 359 plasma
samples from the DELCODE cohort reveals lower oligomer
concentrations in subjective cognitive decline and AD
patients than healthy Control participants.Correlation
analysis between CSF and plasma oligomer concentrations
indicates an impaired clearance of Aβ oligomers that is
dependent on the ApoE ε4 status.},
cin = {AG Peters / AG Dirnagl / AG Endres / AG Düzel / AG Priller
/ AG Schneider / Patient Studies (Bonn) / AG Wiltfang /
Clinical Research (Munich) / AG Dichgans / AG Teipel / AG
Gasser / AG Spottke / Clinical Research Platform (CRP) / AG
Wagner / AG Jessen / AG Schmid Bonn / AG Heneka},
cid = {I:(DE-2719)5000000 / I:(DE-2719)1810002 /
I:(DE-2719)1811005 / I:(DE-2719)5000006 / I:(DE-2719)5000007
/ I:(DE-2719)1011305 / I:(DE-2719)1011101 /
I:(DE-2719)1410006 / I:(DE-2719)1111015 / I:(DE-2719)5000022
/ I:(DE-2719)1510100 / I:(DE-2719)1210000 /
I:(DE-2719)1011103 / I:(DE-2719)1011401 / I:(DE-2719)1011201
/ I:(DE-2719)1011102 / I:(DE-2719)1013028 /
I:(DE-2719)1011303},
pnm = {353 - Clinical and Health Care Research (POF4-353)},
pid = {G:(DE-HGF)POF4-353},
typ = {PUB:(DE-HGF)16},
pmc = {pmc:PMC11631981},
pubmed = {pmid:39658587},
doi = {10.1038/s43856-024-00690-w},
url = {https://pub.dzne.de/record/273930},
}