TY  - JOUR
AU  - Deng, Yushuang
AU  - Liu, Ting
AU  - Scifo, Enzo
AU  - Li, Tao
AU  - Xie, Kan
AU  - Taschler, Bernd
AU  - Morsy, Sarah
AU  - Schaaf, Kristina
AU  - Ehninger, Armin
AU  - Bano, Daniele
AU  - Ehninger, Dan
TI  - Analysis of the senescence-associated cell surfaceome reveals potential senotherapeutic targets.
JO  - Aging cell
VL  - 23
IS  - 12
SN  - 1474-9718
CY  - Oxford [u.a.]
PB  - Wiley-Blackwell
M1  - DZNE-2024-01411
SP  - e14312
PY  - 2024
AB  - The accumulation of senescent cells is thought to play a crucial role in aging-associated physiological decline and the pathogenesis of various age-related pathologies. Targeting senescence-associated cell surface molecules through immunotherapy emerges as a promising avenue for the selective removal of these cells. Despite its potential, a thorough characterization of senescence-specific surface proteins remains to be achieved. Our study addresses this gap by conducting an extensive analysis of the cell surface proteome, or 'surfaceome', in senescent cells, spanning various senescence induction regimes and encompassing both murine and human cell types. Utilizing quantitative mass spectrometry, we investigated enriched cell surface proteins across eight distinct models of senescence. Our results uncover significant changes in surfaceome expression profiles during senescence, highlighting extensive modifications in cell mechanics and extracellular matrix remodeling. Our research also reveals substantive heterogeneity of senescence, predominantly influenced by cell type and senescence inducer. A key discovery of our study is the identification of four unique cell surface proteins with extracellular epitopes. These proteins are expressed in senescent cells, absent or present at low levels in their proliferating counterparts, and notably upregulated in tissues from aged mice and an Alzheimer's disease mouse model. These proteins stand out as promising candidates for senotherapeutic targeting, offering potential pathways for the detection and strategic targeting of senescent cell populations in aging and age-related diseases.
KW  - Cellular Senescence: drug effects
KW  - Animals
KW  - Humans
KW  - Mice
KW  - Senotherapeutics: pharmacology
KW  - Aging: metabolism
KW  - Membrane Proteins: metabolism
KW  - aging (Other)
KW  - cell surface proteins (Other)
KW  - cellular senescence (Other)
KW  - mass spectrometry (Other)
KW  - senotherapeutics (Other)
KW  - Senotherapeutics (NLM Chemicals)
KW  - Membrane Proteins (NLM Chemicals)
LB  - PUB:(DE-HGF)16
C6  - pmid:39228130
C2  - pmc:PMC11634743
DO  - DOI:10.1111/acel.14312
UR  - https://pub.dzne.de/record/273937
ER  -