000273978 001__ 273978
000273978 005__ 20250127091347.0
000273978 0247_ $$2pmid$$apmid:39760355
000273978 0247_ $$2doi$$a10.3791/67100
000273978 037__ $$aDZNE-2024-01427
000273978 041__ $$aEnglish
000273978 082__ $$a570
000273978 1001_ $$aDrude, Natascha$$b0
000273978 245__ $$aOperating and Biocontainment Procedures of a Facility for Laboratory Mice with a Natural Microbiome: Immunophenotyping Procedure
000273978 260__ $$aCambridge, MA$$bJoVE$$c2024
000273978 3367_ $$2DRIVER$$aarticle
000273978 3367_ $$2DataCite$$aOutput Types/Journal article
000273978 3367_ $$0PUB:(DE-HGF)16$$2PUB:(DE-HGF)$$aJournal Article$$bjournal$$mjournal$$s1736760000_13802
000273978 3367_ $$2BibTeX$$aARTICLE
000273978 3367_ $$2ORCID$$aJOURNAL_ARTICLE
000273978 3367_ $$00$$2EndNote$$aJournal Article
000273978 500__ $$aISSN 1940-087X not unique: **10 hits**.
000273978 520__ $$a The use of laboratory mice with a natural microbiome, such as 'Wildling mice', offers a promising research tool for both basic and applied science due to their close resemblance to the human superorganism. However, the breeding and maintenance of these mice, which harbor a diverse microbiome including bacteria, viruses, and parasites, pose significant challenges for animal husbandry facilities at research institutions. To address these challenges, a specialized facility concept was developed for housing 'Wildling mice' at Charité - Universitätsmedizin Berlin. This approach involved designing a facility with specific structural features and operational protocols to effectively contain the natural microbiome, thereby protecting areas with higher hygiene standards. A methodology for blood sampling from both specified pathogen-free (SPF) and 'Wildling mice' for immunophenotyping is demonstrated, highlighting the workflow and biocontainment measures implemented in the facility. Remarkable results reveal that 'Wildling mice' exposed to a natural microbiome develop distinct immune cell populations, which are significantly reduced in mice bred and maintained under stringent hygiene conditions. The significance of this study lies in its potential to provide researchers with access to mice that possess a natural microbiome and a mature immune system similar to that of human adults. This approach could enhance the translatability of preclinical findings into clinical practice, thereby advancing the field of biomedical research.
000273978 536__ $$0G:(DE-HGF)POF4-353$$a353 - Clinical and Health Care Research (POF4-353)$$cPOF4-353$$fPOF IV$$x0
000273978 588__ $$aDataset connected to CrossRef, Journals: pub.dzne.de
000273978 650_2 $$2MeSH$$aAnimals
000273978 650_2 $$2MeSH$$aMice
000273978 650_2 $$2MeSH$$aMicrobiota: immunology
000273978 650_2 $$2MeSH$$aImmunophenotyping: methods
000273978 650_2 $$2MeSH$$aContainment of Biohazards: methods
000273978 650_2 $$2MeSH$$aAnimal Husbandry: methods
000273978 650_2 $$2MeSH$$aHousing, Animal
000273978 7001_ $$aDiederich, Kai$$b1
000273978 7001_ $$aDuerr, Claudia U.$$b2
000273978 7001_ $$aHaase, Nadine$$b3
000273978 7001_ $$aHarms, Christoph$$b4
000273978 7001_ $$0P:(DE-2719)2812386$$aHeppner, Frank$$b5$$udzne
000273978 7001_ $$0P:(DE-HGF)0$$aJendrach, Marina$$b6
000273978 7001_ $$aKahnau, Pia$$b7
000273978 7001_ $$aKolesnichenko, Marina$$b8
000273978 7001_ $$aLewejohann, Lars$$b9
000273978 7001_ $$aKurreck, Claudia$$b10
000273978 7001_ $$aLohan, Anke$$b11
000273978 7001_ $$aMall, Marcus A.$$b12
000273978 7001_ $$aMüller, Dominik$$b13
000273978 7001_ $$aNagel-Riedasch, Stefan$$b14
000273978 7001_ $$aOpitz, Bastian$$b15
000273978 7001_ $$aSchaupp, Laura$$b16
000273978 7001_ $$aSchönfelder, Gilbert$$b17
000273978 7001_ $$aWeber, Amelie$$b18
000273978 7001_ $$aWillimsky, Gerald$$b19
000273978 7001_ $$aZang, Yalei$$b20
000273978 7001_ $$aRosshart, Stephan P.$$b21
000273978 7001_ $$aDiefenbach, Andreas$$b22
000273978 7001_ $$aJordan, Stefan$$b23
000273978 773__ $$0PERI:(DE-600)2975414-8$$a10.3791/67100$$gno. 214, p. 67100$$n214$$p67100$$tJoVE science education$$vImmunology and Infection$$x1940-087X$$y2024
000273978 909CO $$ooai:pub.dzne.de:273978$$pVDB
000273978 9101_ $$0I:(DE-588)1065079516$$6P:(DE-2719)2812386$$aDeutsches Zentrum für Neurodegenerative Erkrankungen$$b5$$kDZNE
000273978 9101_ $$0I:(DE-588)1065079516$$6P:(DE-HGF)0$$aDeutsches Zentrum für Neurodegenerative Erkrankungen$$b6$$kDZNE
000273978 9131_ $$0G:(DE-HGF)POF4-353$$1G:(DE-HGF)POF4-350$$2G:(DE-HGF)POF4-300$$3G:(DE-HGF)POF4$$4G:(DE-HGF)POF$$aDE-HGF$$bGesundheit$$lNeurodegenerative Diseases$$vClinical and Health Care Research$$x0
000273978 9141_ $$y2024
000273978 915__ $$0StatID:(DE-HGF)0100$$2StatID$$aJCR$$bJOVE-J VIS EXP : 2019$$d2021-05-04
000273978 915__ $$0StatID:(DE-HGF)0200$$2StatID$$aDBCoverage$$bSCOPUS$$d2021-05-04
000273978 915__ $$0StatID:(DE-HGF)0300$$2StatID$$aDBCoverage$$bMedline$$d2021-05-04
000273978 915__ $$0StatID:(DE-HGF)0320$$2StatID$$aDBCoverage$$bPubMed Central$$d2021-05-04
000273978 915__ $$0StatID:(DE-HGF)0199$$2StatID$$aDBCoverage$$bClarivate Analytics Master Journal List$$d2021-05-04
000273978 915__ $$0StatID:(DE-HGF)0160$$2StatID$$aDBCoverage$$bEssential Science Indicators$$d2021-05-04
000273978 915__ $$0StatID:(DE-HGF)1050$$2StatID$$aDBCoverage$$bBIOSIS Previews$$d2021-05-04
000273978 915__ $$0StatID:(DE-HGF)1190$$2StatID$$aDBCoverage$$bBiological Abstracts$$d2021-05-04
000273978 915__ $$0StatID:(DE-HGF)0113$$2StatID$$aWoS$$bScience Citation Index Expanded$$d2021-05-04
000273978 915__ $$0StatID:(DE-HGF)0150$$2StatID$$aDBCoverage$$bWeb of Science Core Collection$$d2021-05-04
000273978 915__ $$0StatID:(DE-HGF)9900$$2StatID$$aIF < 5$$d2021-05-04
000273978 9201_ $$0I:(DE-2719)1810007$$kAG Heppner$$lNeuroimmunology$$x0
000273978 980__ $$ajournal
000273978 980__ $$aVDB
000273978 980__ $$aI:(DE-2719)1810007
000273978 980__ $$aUNRESTRICTED