% IMPORTANT: The following is UTF-8 encoded.  This means that in the presence
% of non-ASCII characters, it will not work with BibTeX 0.99 or older.
% Instead, you should use an up-to-date BibTeX implementation like “bibtex8” or
% “biber”.

@ARTICLE{Drude:273978,
      author       = {Drude, Natascha and Diederich, Kai and Duerr, Claudia U.
                      and Haase, Nadine and Harms, Christoph and Heppner, Frank
                      and Jendrach, Marina and Kahnau, Pia and Kolesnichenko,
                      Marina and Lewejohann, Lars and Kurreck, Claudia and Lohan,
                      Anke and Mall, Marcus A. and Müller, Dominik and
                      Nagel-Riedasch, Stefan and Opitz, Bastian and Schaupp, Laura
                      and Schönfelder, Gilbert and Weber, Amelie and Willimsky,
                      Gerald and Zang, Yalei and Rosshart, Stephan P. and
                      Diefenbach, Andreas and Jordan, Stefan},
      title        = {{O}perating and {B}iocontainment {P}rocedures of a
                      {F}acility for {L}aboratory {M}ice with a {N}atural
                      {M}icrobiome: {I}mmunophenotyping {P}rocedure},
      journal      = {JoVE science education},
      volume       = {Immunology and Infection},
      number       = {214},
      issn         = {1940-087X},
      address      = {Cambridge, MA},
      publisher    = {JoVE},
      reportid     = {DZNE-2024-01427},
      pages        = {67100},
      year         = {2024},
      note         = {ISSN 1940-087X not unique: **10 hits**.},
      abstract     = {The use of laboratory mice with a natural microbiome, such
                      as 'Wildling mice', offers a promising research tool for
                      both basic and applied science due to their close
                      resemblance to the human superorganism. However, the
                      breeding and maintenance of these mice, which harbor a
                      diverse microbiome including bacteria, viruses, and
                      parasites, pose significant challenges for animal husbandry
                      facilities at research institutions. To address these
                      challenges, a specialized facility concept was developed for
                      housing 'Wildling mice' at Charité - Universitätsmedizin
                      Berlin. This approach involved designing a facility with
                      specific structural features and operational protocols to
                      effectively contain the natural microbiome, thereby
                      protecting areas with higher hygiene standards. A
                      methodology for blood sampling from both specified
                      pathogen-free (SPF) and 'Wildling mice' for
                      immunophenotyping is demonstrated, highlighting the workflow
                      and biocontainment measures implemented in the facility.
                      Remarkable results reveal that 'Wildling mice' exposed to a
                      natural microbiome develop distinct immune cell populations,
                      which are significantly reduced in mice bred and maintained
                      under stringent hygiene conditions. The significance of this
                      study lies in its potential to provide researchers with
                      access to mice that possess a natural microbiome and a
                      mature immune system similar to that of human adults. This
                      approach could enhance the translatability of preclinical
                      findings into clinical practice, thereby advancing the field
                      of biomedical research.},
      keywords     = {Animals / Mice / Microbiota: immunology /
                      Immunophenotyping: methods / Containment of Biohazards:
                      methods / Animal Husbandry: methods / Housing, Animal},
      cin          = {AG Heppner},
      ddc          = {570},
      cid          = {I:(DE-2719)1810007},
      pnm          = {353 - Clinical and Health Care Research (POF4-353)},
      pid          = {G:(DE-HGF)POF4-353},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:39760355},
      doi          = {10.3791/67100},
      url          = {https://pub.dzne.de/record/273978},
}