%0 Electronic Article
%A Fink, Ryan
%A Imai, Shosei
%A Gockel, Nala
%A Lauer, German
%A Renken, Kim
%A Wietek, Jonas
%A Lamothe-Molina, Paul J
%A Furhmann, Falko
%A Mittag, Manuel
%A Ziebarth, Tim
%A Canziani, Annika
%A Kubitschke, Martin
%A Kistmacher, Vivien
%A Kretschmer, Anny
%A Sebastian, Eva
%A Schmitz, Dietmar
%A Terai, Takuya
%A Gründemann, Jan
%A Hassan, Sami
%A Patriarchi, Tommaso
%A Reiner, Andreas
%A Fuhrmann, Martin
%A Campbell, Robert E
%A Masseck, Olivia A
%T PinkyCaMP a mScarlet-based calcium sensor with exceptional brightness, photostability, and multiplexing capabilities
%J bioRxiv
%M DZNE-2025-00068
%D 2024
%X Genetically encoded calcium (Ca2+) indicators (GECIs) are widely used for imaging neuronal activity, yet current limitations of existing red fluorescent GECIs have constrained their applicability. The inherently dim fluorescence and low signal-to-noise ratio of red-shifted GECIs have posed significant challenges. More critically, several red-fluorescent GECIs exhibit photoswitching when exposed to blue light, thereby limiting their applicability in all-optical experimental approaches. Here, we present the development of PinkyCaMP, the first mScarlet-based Ca2+ sensor that outperforms current red fluorescent sensors in brightness, photostability, signal-to-noise ratio, and compatibility with optogenetics and neurotransmitter imaging. PinkyCaMP is well-tolerated by neurons, showing no toxicity or aggregation, both in vitro and in vivo. All imaging approaches, including single-photon excitation methods such as fiber photometry, widefield imaging, miniscope imaging, as well as two-photon imaging in awake mice, are fully compatible with PinkyCaMP.
%F PUB:(DE-HGF)25
%9 Preprint
%$ pmid:39763884
%R 10.1101/2024.12.16.628673
%U https://pub.dzne.de/record/275833