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000275833 0247_ $$2doi$$a10.1101/2024.12.16.628673
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000275833 037__ $$aDZNE-2025-00068
000275833 1001_ $$00009-0001-2661-5586$$aFink, Ryan$$b0
000275833 245__ $$aPinkyCaMP a mScarlet-based calcium sensor with exceptional brightness, photostability, and multiplexing capabilities
000275833 260__ $$c2024
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000275833 520__ $$aGenetically encoded calcium (Ca2+) indicators (GECIs) are widely used for imaging neuronal activity, yet current limitations of existing red fluorescent GECIs have constrained their applicability. The inherently dim fluorescence and low signal-to-noise ratio of red-shifted GECIs have posed significant challenges. More critically, several red-fluorescent GECIs exhibit photoswitching when exposed to blue light, thereby limiting their applicability in all-optical experimental approaches. Here, we present the development of PinkyCaMP, the first mScarlet-based Ca2+ sensor that outperforms current red fluorescent sensors in brightness, photostability, signal-to-noise ratio, and compatibility with optogenetics and neurotransmitter imaging. PinkyCaMP is well-tolerated by neurons, showing no toxicity or aggregation, both in vitro and in vivo. All imaging approaches, including single-photon excitation methods such as fiber photometry, widefield imaging, miniscope imaging, as well as two-photon imaging in awake mice, are fully compatible with PinkyCaMP.
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000275833 7001_ $$00000-0003-0388-4764$$aImai, Shosei$$b1
000275833 7001_ $$0P:(DE-2719)9002002$$aGockel, Nala$$b2
000275833 7001_ $$00000-0002-4588-2743$$aLauer, German$$b3
000275833 7001_ $$00009-0004-4644-7673$$aRenken, Kim$$b4
000275833 7001_ $$00000-0002-8079-7042$$aWietek, Jonas$$b5
000275833 7001_ $$00000-0002-7793-5133$$aLamothe-Molina, Paul J$$b6
000275833 7001_ $$0P:(DE-HGF)0$$aFurhmann, Falko$$b7
000275833 7001_ $$0P:(DE-2719)2811044$$aMittag, Manuel$$b8
000275833 7001_ $$0P:(DE-HGF)0$$aZiebarth, Tim$$b9
000275833 7001_ $$00009-0006-4051-8615$$aCanziani, Annika$$b10
000275833 7001_ $$00009-0009-1212-0361$$aKubitschke, Martin$$b11
000275833 7001_ $$0P:(DE-HGF)0$$aKistmacher, Vivien$$b12
000275833 7001_ $$0P:(DE-2719)2810979$$aKretschmer, Anny$$b13
000275833 7001_ $$0P:(DE-2719)9002055$$aSebastian, Eva$$b14
000275833 7001_ $$0P:(DE-2719)2810725$$aSchmitz, Dietmar$$b15$$udzne
000275833 7001_ $$00000-0002-3425-3589$$aTerai, Takuya$$b16
000275833 7001_ $$0P:(DE-2719)9001219$$aGründemann, Jan$$b17
000275833 7001_ $$00000-0002-8784-143X$$aHassan, Sami$$b18
000275833 7001_ $$00000-0001-9351-3734$$aPatriarchi, Tommaso$$b19
000275833 7001_ $$00000-0003-0802-7278$$aReiner, Andreas$$b20
000275833 7001_ $$0P:(DE-2719)2679991$$aFuhrmann, Martin$$b21
000275833 7001_ $$00000-0003-0604-092X$$aCampbell, Robert E$$b22
000275833 7001_ $$00000-0002-1541-2388$$aMasseck, Olivia A$$b23
000275833 773__ $$a10.1101/2024.12.16.628673$$tbioRxiv$$y2024
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000275833 9201_ $$0I:(DE-2719)5000069$$kAG Gründemann$$lNeural Circuit Computations$$x0
000275833 9201_ $$0I:(DE-2719)1011004$$kAG Fuhrmann$$lNeuroimmunology and Imaging$$x1
000275833 9201_ $$0I:(DE-2719)1810004$$kAG Schmitz$$lNetwork Dysfunction$$x2
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