TY  - EJOUR
AU  - Fink, Ryan
AU  - Imai, Shosei
AU  - Gockel, Nala
AU  - Lauer, German
AU  - Renken, Kim
AU  - Wietek, Jonas
AU  - Lamothe-Molina, Paul J
AU  - Furhmann, Falko
AU  - Mittag, Manuel
AU  - Ziebarth, Tim
AU  - Canziani, Annika
AU  - Kubitschke, Martin
AU  - Kistmacher, Vivien
AU  - Kretschmer, Anny
AU  - Sebastian, Eva
AU  - Schmitz, Dietmar
AU  - Terai, Takuya
AU  - Gründemann, Jan
AU  - Hassan, Sami
AU  - Patriarchi, Tommaso
AU  - Reiner, Andreas
AU  - Fuhrmann, Martin
AU  - Campbell, Robert E
AU  - Masseck, Olivia A
TI  - PinkyCaMP a mScarlet-based calcium sensor with exceptional brightness, photostability, and multiplexing capabilities
JO  - bioRxiv
M1  - DZNE-2025-00068
PY  - 2024
AB  - Genetically encoded calcium (Ca2+) indicators (GECIs) are widely used for imaging neuronal activity, yet current limitations of existing red fluorescent GECIs have constrained their applicability. The inherently dim fluorescence and low signal-to-noise ratio of red-shifted GECIs have posed significant challenges. More critically, several red-fluorescent GECIs exhibit photoswitching when exposed to blue light, thereby limiting their applicability in all-optical experimental approaches. Here, we present the development of PinkyCaMP, the first mScarlet-based Ca2+ sensor that outperforms current red fluorescent sensors in brightness, photostability, signal-to-noise ratio, and compatibility with optogenetics and neurotransmitter imaging. PinkyCaMP is well-tolerated by neurons, showing no toxicity or aggregation, both in vitro and in vivo. All imaging approaches, including single-photon excitation methods such as fiber photometry, widefield imaging, miniscope imaging, as well as two-photon imaging in awake mice, are fully compatible with PinkyCaMP.
LB  - PUB:(DE-HGF)25
C6  - pmid:39763884
DO  - DOI:10.1101/2024.12.16.628673
UR  - https://pub.dzne.de/record/275833
ER  -