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@ARTICLE{Fink:275833,
author = {Fink, Ryan and Imai, Shosei and Gockel, Nala and Lauer,
German and Renken, Kim and Wietek, Jonas and Lamothe-Molina,
Paul J and Furhmann, Falko and Mittag, Manuel and Ziebarth,
Tim and Canziani, Annika and Kubitschke, Martin and
Kistmacher, Vivien and Kretschmer, Anny and Sebastian, Eva
and Schmitz, Dietmar and Terai, Takuya and Gründemann, Jan
and Hassan, Sami and Patriarchi, Tommaso and Reiner, Andreas
and Fuhrmann, Martin and Campbell, Robert E and Masseck,
Olivia A},
title = {{P}inky{C}a{MP} a m{S}carlet-based calcium sensor with
exceptional brightness, photostability, and multiplexing
capabilities},
journal = {bioRxiv},
reportid = {DZNE-2025-00068},
year = {2024},
abstract = {Genetically encoded calcium (Ca2+) indicators (GECIs) are
widely used for imaging neuronal activity, yet current
limitations of existing red fluorescent GECIs have
constrained their applicability. The inherently dim
fluorescence and low signal-to-noise ratio of red-shifted
GECIs have posed significant challenges. More critically,
several red-fluorescent GECIs exhibit photoswitching when
exposed to blue light, thereby limiting their applicability
in all-optical experimental approaches. Here, we present the
development of PinkyCaMP, the first mScarlet-based Ca2+
sensor that outperforms current red fluorescent sensors in
brightness, photostability, signal-to-noise ratio, and
compatibility with optogenetics and neurotransmitter
imaging. PinkyCaMP is well-tolerated by neurons, showing no
toxicity or aggregation, both in vitro and in vivo. All
imaging approaches, including single-photon excitation
methods such as fiber photometry, widefield imaging,
miniscope imaging, as well as two-photon imaging in awake
mice, are fully compatible with PinkyCaMP.},
cin = {AG Gründemann / AG Fuhrmann / AG Schmitz},
cid = {I:(DE-2719)5000069 / I:(DE-2719)1011004 /
I:(DE-2719)1810004},
pnm = {351 - Brain Function (POF4-351)},
pid = {G:(DE-HGF)POF4-351},
typ = {PUB:(DE-HGF)25},
pubmed = {pmid:39763884},
doi = {10.1101/2024.12.16.628673},
url = {https://pub.dzne.de/record/275833},
}