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| Home > Publications Database > PinkyCaMP a mScarlet-based calcium sensor with exceptional brightness, photostability, and multiplexing capabilities > print |
| 001 | 275833 | ||
| 005 | 20250119000333.0 | ||
| 024 | 7 | _ | |a 10.1101/2024.12.16.628673 |2 doi |
| 024 | 7 | _ | |a altmetric:172155388 |2 altmetric |
| 024 | 7 | _ | |a pmid:39763884 |2 pmid |
| 037 | _ | _ | |a DZNE-2025-00068 |
| 100 | 1 | _ | |a Fink, Ryan |0 0009-0001-2661-5586 |b 0 |
| 245 | _ | _ | |a PinkyCaMP a mScarlet-based calcium sensor with exceptional brightness, photostability, and multiplexing capabilities |
| 260 | _ | _ | |c 2024 |
| 336 | 7 | _ | |a Preprint |b preprint |m preprint |0 PUB:(DE-HGF)25 |s 1736941433_13620 |2 PUB:(DE-HGF) |
| 336 | 7 | _ | |a WORKING_PAPER |2 ORCID |
| 336 | 7 | _ | |a Electronic Article |0 28 |2 EndNote |
| 336 | 7 | _ | |a preprint |2 DRIVER |
| 336 | 7 | _ | |a ARTICLE |2 BibTeX |
| 336 | 7 | _ | |a Output Types/Working Paper |2 DataCite |
| 520 | _ | _ | |a Genetically encoded calcium (Ca2+) indicators (GECIs) are widely used for imaging neuronal activity, yet current limitations of existing red fluorescent GECIs have constrained their applicability. The inherently dim fluorescence and low signal-to-noise ratio of red-shifted GECIs have posed significant challenges. More critically, several red-fluorescent GECIs exhibit photoswitching when exposed to blue light, thereby limiting their applicability in all-optical experimental approaches. Here, we present the development of PinkyCaMP, the first mScarlet-based Ca2+ sensor that outperforms current red fluorescent sensors in brightness, photostability, signal-to-noise ratio, and compatibility with optogenetics and neurotransmitter imaging. PinkyCaMP is well-tolerated by neurons, showing no toxicity or aggregation, both in vitro and in vivo. All imaging approaches, including single-photon excitation methods such as fiber photometry, widefield imaging, miniscope imaging, as well as two-photon imaging in awake mice, are fully compatible with PinkyCaMP. |
| 536 | _ | _ | |a 351 - Brain Function (POF4-351) |0 G:(DE-HGF)POF4-351 |c POF4-351 |f POF IV |x 0 |
| 588 | _ | _ | |a Dataset connected to CrossRef |
| 700 | 1 | _ | |a Imai, Shosei |0 0000-0003-0388-4764 |b 1 |
| 700 | 1 | _ | |a Gockel, Nala |0 P:(DE-2719)9002002 |b 2 |
| 700 | 1 | _ | |a Lauer, German |0 0000-0002-4588-2743 |b 3 |
| 700 | 1 | _ | |a Renken, Kim |0 0009-0004-4644-7673 |b 4 |
| 700 | 1 | _ | |a Wietek, Jonas |0 0000-0002-8079-7042 |b 5 |
| 700 | 1 | _ | |a Lamothe-Molina, Paul J |0 0000-0002-7793-5133 |b 6 |
| 700 | 1 | _ | |a Furhmann, Falko |0 P:(DE-HGF)0 |b 7 |
| 700 | 1 | _ | |a Mittag, Manuel |0 P:(DE-2719)2811044 |b 8 |
| 700 | 1 | _ | |a Ziebarth, Tim |0 P:(DE-HGF)0 |b 9 |
| 700 | 1 | _ | |a Canziani, Annika |0 0009-0006-4051-8615 |b 10 |
| 700 | 1 | _ | |a Kubitschke, Martin |0 0009-0009-1212-0361 |b 11 |
| 700 | 1 | _ | |a Kistmacher, Vivien |0 P:(DE-HGF)0 |b 12 |
| 700 | 1 | _ | |a Kretschmer, Anny |0 P:(DE-2719)2810979 |b 13 |
| 700 | 1 | _ | |a Sebastian, Eva |0 P:(DE-2719)9002055 |b 14 |
| 700 | 1 | _ | |a Schmitz, Dietmar |0 P:(DE-2719)2810725 |b 15 |u dzne |
| 700 | 1 | _ | |a Terai, Takuya |0 0000-0002-3425-3589 |b 16 |
| 700 | 1 | _ | |a Gründemann, Jan |0 P:(DE-2719)9001219 |b 17 |
| 700 | 1 | _ | |a Hassan, Sami |0 0000-0002-8784-143X |b 18 |
| 700 | 1 | _ | |a Patriarchi, Tommaso |0 0000-0001-9351-3734 |b 19 |
| 700 | 1 | _ | |a Reiner, Andreas |0 0000-0003-0802-7278 |b 20 |
| 700 | 1 | _ | |a Fuhrmann, Martin |0 P:(DE-2719)2679991 |b 21 |
| 700 | 1 | _ | |a Campbell, Robert E |0 0000-0003-0604-092X |b 22 |
| 700 | 1 | _ | |a Masseck, Olivia A |0 0000-0002-1541-2388 |b 23 |
| 773 | _ | _ | |a 10.1101/2024.12.16.628673 |y 2024 |t bioRxiv |
| 909 | C | O | |o oai:pub.dzne.de:275833 |p VDB |
| 910 | 1 | _ | |a Deutsches Zentrum für Neurodegenerative Erkrankungen |0 I:(DE-588)1065079516 |k DZNE |b 2 |6 P:(DE-2719)9002002 |
| 910 | 1 | _ | |a Deutsches Zentrum für Neurodegenerative Erkrankungen |0 I:(DE-588)1065079516 |k DZNE |b 7 |6 P:(DE-HGF)0 |
| 910 | 1 | _ | |a Deutsches Zentrum für Neurodegenerative Erkrankungen |0 I:(DE-588)1065079516 |k DZNE |b 8 |6 P:(DE-2719)2811044 |
| 910 | 1 | _ | |a Deutsches Zentrum für Neurodegenerative Erkrankungen |0 I:(DE-588)1065079516 |k DZNE |b 13 |6 P:(DE-2719)2810979 |
| 910 | 1 | _ | |a Deutsches Zentrum für Neurodegenerative Erkrankungen |0 I:(DE-588)1065079516 |k DZNE |b 14 |6 P:(DE-2719)9002055 |
| 910 | 1 | _ | |a External Institute |0 I:(DE-HGF)0 |k Extern |b 15 |6 P:(DE-2719)2810725 |
| 910 | 1 | _ | |a Deutsches Zentrum für Neurodegenerative Erkrankungen |0 I:(DE-588)1065079516 |k DZNE |b 17 |6 P:(DE-2719)9001219 |
| 910 | 1 | _ | |a Deutsches Zentrum für Neurodegenerative Erkrankungen |0 I:(DE-588)1065079516 |k DZNE |b 21 |6 P:(DE-2719)2679991 |
| 913 | 1 | _ | |a DE-HGF |b Gesundheit |l Neurodegenerative Diseases |1 G:(DE-HGF)POF4-350 |0 G:(DE-HGF)POF4-351 |3 G:(DE-HGF)POF4 |2 G:(DE-HGF)POF4-300 |4 G:(DE-HGF)POF |v Brain Function |x 0 |
| 920 | 1 | _ | |0 I:(DE-2719)5000069 |k AG Gründemann |l Neural Circuit Computations |x 0 |
| 920 | 1 | _ | |0 I:(DE-2719)1011004 |k AG Fuhrmann |l Neuroimmunology and Imaging |x 1 |
| 920 | 1 | _ | |0 I:(DE-2719)1810004 |k AG Schmitz |l Network Dysfunction |x 2 |
| 980 | _ | _ | |a preprint |
| 980 | _ | _ | |a VDB |
| 980 | _ | _ | |a I:(DE-2719)5000069 |
| 980 | _ | _ | |a I:(DE-2719)1011004 |
| 980 | _ | _ | |a I:(DE-2719)1810004 |
| 980 | _ | _ | |a UNRESTRICTED |
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